Single B Cell Antibody Service

Single B cell screening directly accesses naturally B cells from immunized animals. This allows you obtain high-affinity, highly specific antibodies since they maintain their natural pairing of heavy and light chains. Often single B cell screening has faster timelines and broader epitope coverage than hybridoma or phage display methods. Unlike hybridomas, there is no need for cell fusion, and unlike phage display, antibodies retain natural pairing of heavy and light chains, ensuring functional binding.

Our single B cell platform captures a broad and diverse repertoire of antibodies because it allows the isolation and characterization of individual B cells, each expressing a unique antibody. Antibodies obtained typically have nanomolar to picomolar affinities, high specificity, and recognize a wide range of epitopes, including challenging or conformational targets. This makes them suitable for diagnostic, therapeutic, and research applications.

Yes. As part of ProteoGenix’s single B cell service, we provide full antibody sequencing, cloning, and transient expression, delivering functional antibodies ready for screening, assay development, or downstream applications.

Absolutely. High-throughput single B cell sorting allows the isolation of rare, high-affinity antibodies that may be difficult to obtain with other methods since it enables the screening and analysis of thousands to millions of individual B cells rapidly and precisely. This makes it especially effective for low-immunogenic, highly conserved, or conformationally complex targets.

Our platform can screen hundreds to thousands of individual B cells, depending on your project. ProteoGenix’s single B cell platform provides at least 30 promising clones that you can validate in your lab to identify the most high-quality antibody candidates. This allows you to select the best-performing clones for application needs.