Recombinant Human CAVIN3 Protein, N-His-SUMO & C-Strep

Description of Recombinant Human CAVIN3 Protein, N-His-SUMO & C-Strep

Introduction

Recombinant Human CAVIN3 protein, also known as Caveolae-associated protein 3, is a 31 kDa protein that plays a crucial role in the formation and maintenance of caveolae, small invaginations of the plasma membrane involved in various cellular processes. This protein is encoded by the CAVIN3 gene and is found in various tissues, including muscle, adipose tissue, and endothelial cells. In this article, we will discuss the structure, activity, and application of Recombinant Human CAVIN3 protein.

Structure of Recombinant Human CAVIN3 Protein

The CAVIN3 gene encodes a 288 amino acid protein that contains a caveolin-binding domain and a coiled-coil domain. The caveolin-binding domain is responsible for the interaction between CAVIN3 and caveolin, a structural protein found in caveolae. The coiled-coil domain is involved in protein-protein interactions and is essential for the formation of high-order oligomers of CAVIN3.

Recombinant Human CAVIN3 protein is produced through genetic engineering techniques, where the CAVIN3 gene is inserted into a suitable expression vector and then expressed in a host cell, typically E. coli or mammalian cells. The resulting protein is purified and can be used in various applications.

Activity of Recombinant Human CAVIN3 Protein

CAVIN3 is a key regulator of caveolae formation and is involved in the assembly and stabilization of caveolae. It interacts with caveolin and other cavin proteins to form a complex that is essential for the structure and function of caveolae. CAVIN3 also plays a role in the regulation of lipid metabolism and signaling pathways, making it a crucial protein in cellular processes.

Recombinant Human CAVIN3 protein can be used to study the role of CAVIN3 in caveolae formation and function. It can also be used to investigate the interaction between CAVIN3 and other caveolae-associated proteins. Additionally, CAVIN3 has been shown to play a role in various diseases, such as cancer and metabolic disorders, and recombinant CAVIN3 protein can be used in research to understand its involvement in these diseases.

Application of Recombinant Human CAVIN3 Protein

Recombinant Human CAVIN3 protein has various applications in both basic research and drug development. It can be used as an antigen in antibody production for the detection of CAVIN3 in tissues or cell lysates. It can also be used in in vitro studies to investigate the role of CAVIN3 in caveolae formation and function.

Furthermore, recombinant CAVIN3 protein has potential therapeutic applications. As CAVIN3 is involved in lipid metabolism and signaling pathways, it can be a target for drug development in diseases such as obesity and diabetes. Recombinant CAVIN3 protein can be used in drug screening assays to identify compounds that can modulate its activity.

In conclusion, Recombinant Human CAVIN3 protein is an important protein involved in the formation and maintenance of caveolae and has various applications in research and drug development. Its structure and activity make it an essential protein to study in various cellular processes, and its potential therapeutic applications make it a promising target for drug development.