Recombinant Human PSMD13, N-His

Description of Recombinant Human PSMD13, N-His

Introduction

Recombinant proteins have become essential tools in various fields of research, diagnostics, and therapeutics. These proteins are produced by genetically engineering a host organism to express a specific gene of interest. One such recombinant protein is PSMD13, which has gained significant attention in the scientific community due to its unique structure, diverse activity, and potential applications. In this article, we will delve into the details of Recombinant Human PSMD13, providing a comprehensive understanding of its structure, activity, and application.

Structure of Recombinant Human PSMD13

PSMD13, also known as Rpn9 or S9, is a subunit of the 26S proteasome, a large protein complex responsible for degrading damaged or misfolded proteins in the cell. The human PSMD13 gene is located on chromosome 7 and encodes a protein of 241 amino acids. The recombinant form of PSMD13 is produced by expressing the gene in a suitable host organism, typically E. coli, and purifying the protein using various chromatographic techniques.

The crystal structure of human PSMD13 has been determined, revealing a compact globular protein with a central β-sheet surrounded by α-helices. The protein contains a zinc-binding domain, which is essential for its interaction with other subunits of the 26S proteasome. The recombinant form of PSMD13 retains its native structure, making it a useful tool for studying the function of this protein in various cellular processes.

Activity of Recombinant Human PSMD13

PSMD13 is a crucial component of the 26S proteasome, where it plays a vital role in recognizing and binding to ubiquitinated proteins. This binding leads to the degradation of the tagged proteins by the proteasome, ensuring proper protein turnover and maintaining cellular homeostasis. Recombinant Human PSMD13 has been shown to have the same activity as its native form, making it an ideal tool for studying the function of the 26S proteasome in various cellular processes.

Moreover, PSMD13 has been found to interact with other proteins and participate in various signaling pathways. It has been shown to interact with the tumor suppressor protein p53, regulating its stability and activity. PSMD13 has also been implicated in the regulation of the NF-κB signaling pathway, which plays a critical role in inflammation and immune response. These findings suggest that recombinant PSMD13 can be used to study the role of this protein in different cellular processes and diseases.

Application of Recombinant Human PSMD13

The unique structure and activity of PSMD13 make it a valuable tool in various fields of research. Its role in protein degradation and signaling pathways makes it an essential protein in studying diseases such as cancer, neurodegenerative disorders, and autoimmune diseases. Recombinant Human PSMD13 has been used in various studies to investigate the role of the 26S proteasome in cancer progression and to identify potential therapeutic targets.

In addition, recombinant PSMD13 has also been used in drug discovery and development. It has been shown to interact with various small molecules and peptides, making it a potential target for drug design. Furthermore, recombinant PSMD13 can also be used in diagnostic assays, such as ELISAs, to detect the presence of this protein in biological samples.

Conclusion

In summary, Recombinant Human PSMD13 is a valuable tool in scientific research due to its unique structure, diverse activity, and potential applications. Its role in protein degradation and signaling pathways makes it an essential protein in studying various diseases, and its potential as a drug target and diagnostic tool further highlights its significance. With ongoing research, the potential applications of recombinant PS