Recombinant Human SIRT6, N-His

Description of Recombinant Human SIRT6, N-His

Introduction

Recombinant Human SIRT6 is a protein that has been artificially produced in a laboratory using genetic engineering techniques. It is a member of the sirtuin family of proteins, which are known for their role in regulating cellular metabolism and aging. SIRT6 has been studied extensively for its potential therapeutic applications, particularly in the fields of cancer and neurodegenerative diseases.

Structure of Recombinant Human SIRT6

Recombinant Human SIRT6 is a 355 amino acid protein with a molecular weight of approximately 39 kDa. It is composed of several domains, including a NAD+ binding domain, a catalytic domain, and a C-terminal extension. The protein also contains a zinc-binding motif, which is essential for its enzymatic activity.

Activity of Recombinant Human SIRT6

Recombinant Human SIRT6 is a nicotinamide adenine dinucleotide (NAD+)-dependent deacetylase enzyme. It is involved in the regulation of various cellular processes, such as DNA repair, glucose metabolism, and stress response. SIRT6 acts by removing acetyl groups from histone and non-histone proteins, thereby modulating their activity and function.

One of the key functions of SIRT6 is its role in maintaining genomic stability. It has been shown to promote DNA repair by deacetylating histone H3 lysine 9 (H3K9) at DNA double-strand breaks, which allows for the recruitment of repair proteins to the damaged site. This activity of SIRT6 is critical for preventing the accumulation of DNA damage and maintaining the integrity of the genome.

In addition to its role in DNA repair, SIRT6 has also been implicated in regulating glucose metabolism. It has been shown to deacetylate and activate the enzyme phosphoenolpyruvate carboxykinase (PEPCK), which is involved in gluconeogenesis. This activity of SIRT6 helps to maintain glucose homeostasis and prevent the development of metabolic disorders such as diabetes.

Applications of Recombinant Human SIRT6

The unique structure and activity of Recombinant Human SIRT6 make it a promising candidate for therapeutic applications. One potential application is in the treatment of cancer. SIRT6 has been found to play a role in suppressing tumor growth by promoting DNA repair and inhibiting glycolysis, which is a hallmark of cancer cells. Therefore, recombinant SIRT6 could potentially be used to develop targeted therapies for various types of cancer.

In addition, SIRT6 has also been studied for its potential in treating neurodegenerative diseases such as Alzheimer’s and Parkinson’s. It has been shown to protect neurons from oxidative stress and promote their survival. This suggests that recombinant SIRT6 could be used to develop treatments for these debilitating diseases.

Furthermore, the ability of SIRT6 to regulate glucose metabolism makes it a potential target for the treatment of metabolic disorders. By modulating the activity of PEPCK, recombinant SIRT6 could potentially be used to regulate blood glucose levels and prevent the development of diabetes and other metabolic diseases.

Conclusion

In conclusion, Recombinant Human SIRT6 is a protein with a unique structure and activity that holds great potential for therapeutic applications. Its role in DNA repair, glucose metabolism, and stress response make it a promising candidate for the treatment of cancer, neurodegenerative diseases, and metabolic disorders. Further research and development of recombinant SIRT6 could lead to the development of targeted and effective treatments for these diseases.