Recombinant Human CNPY2, N-His

Description of Recombinant Human CNPY2, N-His

Introduction

Recombinant Human CNPY2 is a protein that plays a crucial role in various cellular processes such as protein folding, trafficking and degradation. It is a member of the Canopy FGF signaling regulator (CNPY) family and is highly conserved among different species, including humans. In this article, we will discuss the structure, activity and application of Recombinant Human CNPY2 as a recombinant protein and its potential as an antigen for various research and diagnostic purposes.

Structure of Recombinant Human CNPY2

The human CNPY2 gene is located on chromosome 20 and consists of 7 exons that encode a protein of 135 amino acids. The protein has a molecular weight of approximately 15 kDa and is composed of a signal peptide, a conserved FGF-binding domain, and a C-terminal transmembrane domain. The FGF-binding domain is responsible for the interaction with fibroblast growth factors (FGFs) and plays a crucial role in regulating their signaling pathways.

Recombinant Human CNPY2 is produced through genetic engineering techniques, where the gene is inserted into a suitable expression vector such as E. coli or mammalian cells. The resulting protein is then purified to obtain a highly pure and active form of CNPY2.

Activity of Recombinant Human CNPY2

Recombinant Human CNPY2 has been shown to have a wide range of activities in different cellular processes. One of its main functions is to assist in the proper folding and maturation of proteins in the endoplasmic reticulum (ER). It does this by interacting with chaperone proteins and regulating their activity, thereby ensuring the correct folding and trafficking of newly synthesized proteins.

CNPY2 has also been found to play a role in the degradation of misfolded proteins through the ER-associated degradation (ERAD) pathway. It interacts with components of the ERAD machinery and helps in the recognition and removal of misfolded proteins, thus maintaining the cellular protein quality control.

Moreover, CNPY2 has been shown to regulate the activity of FGFs, which are important growth factors involved in various biological processes such as cell proliferation, differentiation, and survival. CNPY2 binds to FGFs and modulates their signaling pathways, thus playing a crucial role in the development and maintenance of tissues and organs.

Applications of Recombinant Human CNPY2

The unique structure and activity of Recombinant Human CNPY2 make it a valuable tool for various research and diagnostic applications. One of its main applications is in studying protein folding and ER stress-related diseases such as neurodegenerative disorders, cystic fibrosis, and diabetes. CNPY2 has been shown to play a role in these diseases, and its recombinant form can be used to study its mechanisms and potential therapeutic interventions.

Recombinant Human CNPY2 can also be used as an antigen for the production of specific antibodies. Its conserved FGF-binding domain makes it an ideal target for antibody generation, which can then be used for various research and diagnostic purposes. Additionally, CNPY2 antibodies have been shown to have potential as a biomarker for certain diseases, such as prostate cancer.

Furthermore, CNPY2 has been found to be involved in the regulation of immune responses, making it a potential target for immunotherapy. Recombinant Human CNPY2 can be used to study its role in immune modulation and develop therapeutic strategies for autoimmune and inflammatory diseases.

Conclusion

In summary, Recombinant Human CNPY2 is a multifunctional protein with a unique structure and activity. Its recombinant form has various applications in research and diagnostics, including studying protein folding, generating specific antibodies, and developing therapeutic strategies. With its crucial role in cellular processes and potential as a therapeutic target, CNPY2 continues to be a subject of interest in the