Recombinant Human GNAQ Protein, N-His

Description of Recombinant Human GNAQ Protein, N-His

Introduction to Recombinant Human GNAQ Protein

Recombinant Human GNAQ protein, also known as Guanine nucleotide-binding protein G(q) subunit alpha, is a protein that plays a crucial role in signal transduction pathways. This protein is encoded by the GNAQ gene and is a member of the G protein family. Recombinant Human GNAQ protein is widely used in scientific research and has various applications in the field of medicine.

Structure of Recombinant Human GNAQ Protein

Recombinant Human GNAQ protein is a heterotrimeric protein composed of three subunits – alpha, beta, and gamma. The alpha subunit is the main functional component of the protein and is responsible for binding to guanine nucleotides and activating downstream signaling pathways. The beta and gamma subunits are involved in stabilizing the alpha subunit and regulating its activity.

The alpha subunit of Recombinant Human GNAQ protein consists of 354 amino acids and has a molecular weight of approximately 40 kDa. It contains a conserved GTP-binding domain, which is essential for its function. The beta and gamma subunits are smaller in size and have a molecular weight of around 10 kDa.

Activity of Recombinant Human GNAQ Protein

Recombinant Human GNAQ protein is a GTPase enzyme that plays a key role in signal transduction pathways. It is activated by G protein-coupled receptors (GPCRs) upon binding to specific ligands, such as hormones, neurotransmitters, and growth factors. This activation triggers a series of events that ultimately lead to the production of secondary messengers, such as cyclic AMP (cAMP) and inositol triphosphate (IP3).

Recombinant Human GNAQ protein is involved in various cellular processes, including cell proliferation, differentiation, and migration. It also plays a role in regulating ion channels and gene expression. Dysregulation of this protein has been linked to various diseases, such as cancer, cardiovascular disorders, and neurological disorders.

Application of Recombinant Human GNAQ Protein

Recombinant Human GNAQ protein has a wide range of applications in the field of medicine and scientific research. Some of its key applications include:

1. Recombinant Protein Production

Recombinant Human GNAQ protein is widely used in the production of recombinant proteins. It is a crucial component of the G protein-coupled receptor (GPCR) signaling pathway, which is involved in the regulation of protein expression. By using recombinant Human GNAQ protein, researchers can manipulate this pathway to produce large quantities of specific proteins for various applications, such as drug discovery and protein therapeutics.

2. Antigen for Antibody Production

Recombinant Human GNAQ protein can also be used as an antigen to produce antibodies for research and diagnostic purposes. Antibodies against this protein can be used to study its expression, localization, and function in different cell types and tissues. They can also be used to detect any abnormalities in the GPCR signaling pathway, which can be indicative of certain diseases.

3. Drug Target for Disease Treatment

As mentioned earlier, Recombinant Human GNAQ protein has been linked to various diseases. Therefore, it is a potential drug target for the treatment of these diseases. By targeting this protein, researchers can modulate GPCR signaling and potentially treat conditions such as cancer, cardiovascular disorders, and neurological disorders.

4. Research Tool for Signal Transduction Studies

Recombinant Human GNAQ protein is a valuable tool for studying signal transduction pathways. Its role in the GPCR signaling pathway makes it an important target for research on cellular signaling and its dysregulation in diseases. By using recombinant Human GNAQ protein, researchers can gain a better understanding of the mechanisms underlying various cellular processes and develop new therapeutic strategies.

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