Recombinant Human CNR1/CB1 Protein, N-GST & C-His

Description of Recombinant Human CNR1/CB1 Protein, N-GST & C-His

Introduction

Recombinant Human CNR1/CB1 Protein, also known as Cannabinoid Receptor 1 (CB1), is a transmembrane protein that plays a crucial role in the endocannabinoid system. This protein is encoded by the CNR1 gene and is found in various tissues and organs, including the brain, liver, and adipose tissue. CB1 is a G-protein coupled receptor (GPCR) that binds to endogenous cannabinoids, such as anandamide and 2-arachidonoylglycerol, as well as exogenous cannabinoids, including delta-9-tetrahydrocannabinol (THC). In this article, we will discuss the structure, activity, and applications of Recombinant Human CNR1/CB1 Protein.

Structure of Recombinant Human CNR1/CB1 Protein

The CNR1 gene is located on chromosome 6 in humans and consists of 14 exons that encode a 473 amino acid protein. The CB1 protein has seven transmembrane domains, an extracellular N-terminus, and an intracellular C-terminus. The N-terminus contains a glycosylation site, which is important for the proper folding and trafficking of the protein. The C-terminus contains a conserved motif, known as the DRY motif, which is essential for G-protein coupling and activation. The transmembrane domains are responsible for binding to endogenous and exogenous cannabinoids, while the intracellular loops are involved in G-protein signaling.

Activity of Recombinant Human CNR1/CB1 Protein

CB1 is activated by endogenous and exogenous cannabinoids, which leads to a cascade of intracellular signaling events. Upon activation, CB1 interacts with G-proteins, specifically Gαi/o, which results in the inhibition of adenylyl cyclase and subsequent decrease in cAMP levels. This leads to the modulation of various cellular processes, including neurotransmitter release, synaptic plasticity, and gene expression. CB1 is also involved in the regulation of ion channels, such as potassium and calcium channels, which can affect neuronal excitability.

Applications of Recombinant Human CNR1/CB1 Protein

The availability of Recombinant Human CNR1/CB1 Protein has allowed for a better understanding of the structure and function of this receptor. It has also been used in various research studies to investigate the role of CB1 in different physiological and pathological processes. Some of the potential applications of Recombinant Human CNR1/CB1 Protein are discussed below:

1. Drug Discovery: CB1 has been identified as a potential target for the development of therapeutics for various conditions, including pain, inflammation, and neurological disorders. Recombinant Human CNR1/CB1 Protein can be used in high-throughput screening assays to identify novel CB1 ligands with potential therapeutic effects.

2. Neuroscience Research: CB1 is highly expressed in the brain and plays a crucial role in the regulation of neuronal functions. Recombinant Human CNR1/CB1 Protein can be used to study the effects of cannabinoids on neuronal signaling and behavior, as well as the role of CB1 in various neurological disorders.

3. Endocannabinoid System Studies: CB1 is a key component of the endocannabinoid system, which is involved in various physiological processes, including appetite, pain, and mood regulation. Recombinant Human CNR1/CB1 Protein can be used to study the interactions between CB1 and other components of the endocannabinoid system, as well as the role of this system in various diseases.

4. Antibody Production: Recombinant Human CNR1/CB1 Protein can be used as an antigen to generate specific antibodies against CB1. These antibodies can be used in various research and diagnostic applications, such as immunohistochemistry and Western blotting.

Conclusion

In summary, Recombinant Human CNR1/CB1 Protein is a transmembrane protein that plays a crucial role in the endocannabinoid system. It has a unique structure consisting of seven transmembrane domains and is activated by endogenous