Western Blot guaranteed monoclonal antibody production

Monoclonal antibody production package details

Antigen design (design of the most appropriate fragment to be produced)
Two gene syntheses (each gene optimized for a specific expression system) and 2 subclonings
Expression tests in two systems taken from E. coli, B. subtilis, P. pastoris, insect cells and mammalian cells. To be determined by ProteoGenix.
Production of the antigen in the most appropriate expression system depending on the yield and the project requirements.
Mice injections (5 mice)
Immune response control in Western Blot (WB)
Selection of the best two mice for two fusions in order to have a large number and variety of clones
Screening of the fusion product directly in Western Blot (WB): around 1000 lanes tested, 42 WB of 24 lanes
Subcloning of two parental clones (2-3 rounds of limiting dilutions) in Western Blot (WB)
Production and purification of 2mg from the best clone

Level 1 : If we don’t succeed in producing an antibody that detects the immunogen in WB, we charge EUR 1 000
Level 2 : If we succeed in producing an antibody that works in WB against the immunogen, we charge a part of the cost
Level 3 : If after testing the purified antibody the customer wants to receive the hybridoma, we charge the full package price

After total payment: