IHC guaranteed monoclonal antibody production

Monoclonal antibody production package details

Antigen design (design of the most appropriate fragment to be produced)
Two gene syntheses (each gene optimized for a specific expression system) and two subclonings
Expression tests in two systems taken from E. coli, B. subtilis, P. pastoris, insect cells and mammalian cells. To be determined by ProteoGenix.
Production of the antigen in the most appropriate expression system depending on the yield and the project requirements.
Mice injections: three mice with protein produced in native conditions (if available) and three mice with denatured protein.
Immune response control by ELISA
Selection of the best mice of each three mice batch for two fusions in order to have antibodies targeting the native form of the protein and antibodies targeting the denatured form
Screening of the fusion products by ELISA: around 10 x 96-well plates
Subcloning of two parental clones (2-3 rounds of limiting dilutions) examined by ELISA
Production and purification of 2mg from the best clone

Level 1: If we don’t succeed in producing an antibody that works in ELISA against the immunogen, we charge EUR 1000
Level 2: If we succeed in producing an antibody that works in ELISA against the immunogen, we charge a part of the cost
Level 3: If after testing the purified antibody the customer wants to receive the hybridoma, we charge the full package price

After total payment: