Standard ELISA package: ATX-PACK1M
- Mice injections
- Immune response control by ELISA
- Selection of the best mice for fusion
- Screening of the fusion product by ELISA : around 10 x 96-well plates
- Subcloning of 2 parental clones (2-3 rounds of limiting dilutions) examined by ELISA
- If we succeed in producing an antibody that works in ELISA against the immunogen, we charge the full package price
- If we don’t, we charge EUR 800
- 10 -20 best clones at parental stage for customer’s selection
- One hybridoma cell line (four frozen vials)
- 5 ml culture supernatant
- Injections : 7-11 weeks
- Fusion screening:2-4 weeks
- Subcloning: 3-5 weeks
- TOTAL : 12-20 weeks
Premium ELISA guaranteed package: ATX-PACK2M
- Antigen design (design of the most appropriate fragment to be produced)
- Two gene syntheses (each gene optimized for a specific expression system) and 2 subclonings
- Expression tests in two systems taken from E. coli, B. Subtilis, P. Pastoris, insect cells and mammalian cells. To be determined by ProteoGenix.
- Production of the antigen in the most appropriate expression system, depending on the yield and the project requirements.
- Mice injections
- Immune response control by ELISA
- Selection of the two best mice for two fusions, in order to have a large number and variety of clones
- Screening of the fusion product by ELISA: around 10 x 96-well plates
- Subcloning of two parental clones (2-3 rounds of limiting dilutions) examined by ELISA
- Production and purification of 2mg from the best clone
- Level 1: If we don’t succeed in producing an antibody that works in ELISA against the immunogen, we charge EUR 1000
- Level 2 : If we succeed in producing an antibody that works in ELISA against the immunogen, we charge only a small part of the cost
- Level 3 : If after testing the purified antibody the customer wants to receive the hybridoma, we charge the full package price
- 10 -20 best clones at parental stage for customer’s selection
- 100 µg of purified antibody from the best clone to confirm hybridoma purchase
- 50 µg of purified protein
- 1.9 mg of purified antibody
- One hybridoma cell line (four frozen vials)
- 150 µg of purified protein
- Both genes in pUC57
- Gene synthesis: 1-2 weeks
- Protein production : 3-9 weeks
- Injections: 7-11 weeks
- Fusion screening:2-4 weeks
- Subcloning: 3-5 weeks
- Antibody production: 3-4 weeks
- Total: 19-35 weeks
Peptide guaranteed package: ATX-PACK8M
- Two or three peptides: design, synthesis, and conjugation to KLH
- Mice injections with a mix of the peptides
- Immune response control by ELISA
- Selection of the best mice for fusion
- Screening of the fusion product by ELISA : around 10 x 96-well plates
- Subcloning of two parental clones (2-3 rounds of limiting dilutions) examined by Elisa
- If we don’t succeed in producing an antibody that works in ELISA against the peptides, we charge EUR 800
- If we succeed, we charge the full package price
- 10 -20 best clones at parental stage for customer’s selection
- One hybridoma cell line (four frozen vials)
- 2 mg of purified antibody
- Peptide synthesis and conjugation : 3-4 weeks
- Injections: 7-11 weeks
- Fusion screening: 2-4 weeks
- Subcloning: 3-5 weeks
- Antibody production: 3-4 weeks
- Total: 18-28 weeks