Cat Monoclonal Antibody for Phage Display

Are you looking for a high-performing custom cat monoclonal antibody? Look no further than ProteoGenix’s cat antibody phage display service. Simply tell us what feline antigen you would like to target and we will send you three novel antibodies verified to bind your target… in fewer than 7 weeks. Each antibody is ethically sourced using animal-free methods from our prebuilt antibody libraries, ensuring maximized antibody library diversity for identifying high-affinity antibodies. With our extensive libraries and over 300+ successful phage display projects on record, we are confident we can help you obtain the perfect antibody for research, diagnostic, or therapeutic needs. Speak with one of our highly skilled antibody professionals today!

What are the advantages of a Cat Monoclonal Antibody Library

The use of a cat monoclonal antibody library is crucial for precision in researching, diagnosing, and treating specific feline diseases. Any antibody that is required for therapeutic use in felines will need to be isolated from a cat-specific phage display library to avoid immune reactions.

Creating a feline antibody for phage display library involves isolating whole blood samples from affected or healthy cats. Next, the nucleated cells in the blood are isolated and the VH and VL chains are reverse-transcribed into a DNA copy of the mRNA (cDNA) and amplified by PCR. The amplicons are then cloned into a phage display vector, fusing the cDNA to a cell surface phage protein (pIII or pillinIII).

The cat antigen is then used to isolate a unique antibody, using biopanning, from a diverse cat antibody repertoire. Veterinary scientists can use this library to isolate novel monoclonal antibodies for various research, diagnostic, and therapeutic needs. The benefits of a cat antibody library include:

Species Specificity:

• Veterinarians can rely on cat monoclonal antibodies for highly specific results in feline-related studies and diagnostics.
• Ensures accuracy in targeting feline-specific biomarkers, crucial for precise research and diagnostics.

Suitability for Feline Disease Models:

• Facilitates more effective modeling of diseases in cats, enhancing the relevance of research outcomes for veterinary professionals.
• Enables a deeper understanding of diseases such as feline tumors, resulting in improved diagnostic and therapeutic strategies.

Diagnostic Precision:

• Enhances the precision of diagnostic tests, allowing veterinarians to pinpoint and address health issues in cats with confidence.
• Provides reliable tools for early detection and accurate monitoring of feline diseases.

Therapeutic Targeting:

• Offers a promising avenue for developing targeted therapies tailored to feline-specific diseases.
• Fulfills the growing need for innovative and effective treatment options in veterinary medicine.

Reduced Immunogenicity:

• Minimizes the risk of immune reactions, ensuring safer and more effective use of monoclonal antibodies in feline patients.

Versatility in Research:

• Provides researchers with a versatile tool for investigating various aspects of feline immunology, physiology, and pathology.
• Supports a wide range of research applications, fostering innovation in veterinary science.

Vaccine Development:

• Contributes to the development of vaccines for feline diseases, playing a pivotal role in preventive veterinary medicine.

Customization and Engineering:

• Allows for the customization and engineering of antibodies to meet specific research or diagnostic requirements.
• Provides flexibility for veterinarians to adapt antibody characteristics based on the unique demands of their projects.

Availability of Diverse Libraries:

• Offers access to extensive antibody libraries, ensuring a comprehensive toolkit for diverse research and diagnostic needs.
• Provides veterinarians with a broad spectrum of options to address specific challenges in feline health.

Contribution to Comparative Medicine:

• Bridges the gap between veterinary and human medicine, facilitating insights that benefit both fields.
• Supports the broader advancement of medical knowledge, positioning veterinarians at the forefront of comparative medicine.

Our current phage display libraries are built from human, rabbit, dog, and camelid (camel, llama, and alpaca) species. These libraries are perfect for making custom antibodies for research or diagnostic use. However, antibodies used developed into therapeutics would require constructing a naïve cat antibody library to maintain species compatibility and to prevent cross-species immune reactions.

Which ethical advantages for using Cat Antibody Libraries rather than traditional Cat Antibody Production methods?

Conventional methods for developing cat monoclonal antibodies involve subjecting the cat to multiple rounds of immunization and extraction of antibody-producing cells from the cat’s blood. This approach is known to induce stress and discomfort in the feline subjects.

However, ProteoGenix’s antibody phage display strategy offers a more humane and efficient approach to generating cat monoclonal antibodies for the following reasons:

• Reduced Animal Use:
  Cat antibody libraries, especially those generated through phage display, significantly reduce the need for live animals in the antibody production process.

• Elimination of Invasive Procedures:
  Unlike traditional methods involving invasive procedures, library-based approaches prioritize non-invasive techniques, minimizing discomfort and harm to animals.

• Minimized Use of Ascites Fluid:
  The use of cat antibody libraries eliminates or significantly reduces reliance on ascites fluid, sparing animals from the distress associated with this method.

• Humane Endpoints and Control Over Antibody Selection:
  Cat antibody library methods allow for humane endpoints, ensuring that animals are treated ethically and that there’s control over the selection of antibodies without undue harm.

• Ethical Sourcing of Genetic Material:
  The ethical collection of genetic material for library generation ensures a responsible and considerate approach to sourcing biological materials.

• Reduction of Animal Stress:
  Cat antibody libraries contribute to the reduction of overall animal stress, promoting a more compassionate and humane research environment.

• Advancements in In Vitro Technologies:
  By leveraging in vitro technologies, cat antibody libraries represent a progressive shift towards more ethical and efficient cat monoclonal antibody production methods.

What are the different applications of Cat Monoclonal Antibody libraries

Cat monoclonal antibody libraries can be used by veterinary scientists or physicians who:

• want the ability to diagnose complex diseases using antibodies in vitro or in vivo (diagnostics)
• want to develop new biologics to treat diseases with few therapeutic options (therapeutics)
• want to learn more about feline biology (research)

Below are specific applications cat monoclonal antibody libraries can be used for:

• Research in Feline Immunology:
  Antibody sequencing technology offers scientists the ability to identify the structural and functional aspects of cat antibodies. This data can also inform scientists how antibody isoforms and classes change in response to infection or disease.

• Diagnostic Assays:
  Antibody phage display allows for the rapid isolation of highly specific and sensitive antibodies with minimal cross-reactivity making this the preferred method for creating diagnostic antibodies in cats.

• Targeted Therapies for Feline Diseases:
  There has been an explosion of therapeutic monoclonal antibodies to treat human diseases such as cancer. However, more and more pet owners express a desire to treat their companions using these highly specific and effective strategies as well.

• Immunomodulation in Veterinary Medicine:
  Diseases such as feline leukemia, inflammatory bowel disease, and various autoimmune diseases might benefit from antibody-based therapies that can modulate immune cell-activating targets or immune cell-dampening targets.

• Studying Feline Cancers:
  Creating a naïve cat library using animals suffering from a particular feline cancer offers a rich source of antibodies that have undergone natural affinity maturation or selection to tumorigenic protein antigens. The antibodies with high affinity to antigens indispensable for cancer cell survival can then be isolated and developed into a life-saving biologic.

• Infectious Disease Studies:
  Engineering a naïve cat library from infected felines offers a repertoire of pathogen-specific antibodies that have undergone affinity maturation and selection against antigens from the virus, bacteria, or fungi. The antibodies targeting antigens important for pathogen survival can then be isolated and used as therapeutics.

• Phage Display Screening Services:
  Constructing a phage display library from cats puts you in the unique position to offer phage display screening services to individuals or institutions requiring high-affinity feline monoclonal antibodies. You can outsource the services and retain the intellectual property rights.

• Biomedical Imaging:
  Cat monoclonal antibodies identified using antibody phage display work exceptionally well for immunofluorescence and immunohistochemistry research assays. ProteoGenix can also adapt your cat monoclonal antibodies for use in vivo or in vitro diagnostic assays.

• Collaborative Research:
  Owning a cat antibody library for phage display library will launch your lab to the top of your field by being viewed as the go-to source of quality cat monoclonal antibodies supercharging your collaborative potential.

These are just a few examples demonstrating the power of cat monoclonal antibody libraries. Book a free call with one of our antibody experts today and learn how affordably we can make your custom cat monoclonal antibody library.

What is Feline Antibody for Phage Display

The antibody phage display technique is a laboratory approach crafted to explore interactions between antibodies and diverse molecules, including antigens. This involves genetically modifying bacteriophages, viruses infecting bacteria, by merging antibody genes with a coat protein gene of the phage. Through this genetic alteration, the phage exhibits the antibody on its surface while maintaining the antibody gene within the bacteriophage.
This genetic link between genotype and phenotype allows for the identification of antibodies interacting with specific antigens via screening the displayed phages against them. Mimicking natural selection and referred to as in vitro selection, this process facilitates thorough screening and amplification of antibody libraries.

How is a Cat Phage Display Library Made?

The initial phase of constructing a cat antibody library for phage display involves integrating antibody variable region genes into a phage display vector. This begins by extracting mRNA from B-cells obtained from an animal or human patient, followed by converting mRNA into cDNA through reverse transcription. PCR amplification, using antibody cDNA as a template, generates variable regions for both heavy and light chains of each antibody.
Post-amplification, these variable regions are inserted into a modified phage display vector expressing them as a fusion protein with a coat protein on the phage’s surface. This structure allows visible presentation of antibodies on the phage’s exterior, with the DNA encoding the antibody enclosed within the phage particle.
Subsequently, the library undergoes screening against a specific target antigen to identify phages displaying antibodies capable of binding to the target. Successful phages are isolated, allowing for elution, and subsequent DNA sequencing of the antibody-encoding DNA facilitates the cat monoclonal antibody production for further examination or therapeutic applications.

The Difference Between a Cat Naïve Library and a Cat Immune Library

A naive cat monoclonal antibody library for phage display incorporates genes from an organism unexposed to the specific antigen, while an immune library integrates genes from an organism immunized with the antigen. Naive libraries offer diverse antibodies without antigen-driven selection, increasing the potential for unique binding properties.

In contrast, immune libraries contain antibodies chosen for their affinity to the specific antigen, valuable for rapid identification. The primary distinction lies in gene origin: naive libraries encompass a broad spectrum of antibodies unfamiliar with the antigen, whereas immune libraries comprise antibodies selected for their capability to bind to that specific antigen.