Single B Cell Screening Service

Single B-cell sequencing is an efficient monoclonal antibody development and screening strategy which principle is based on the amplification of genes encoding the VH and VL region from B-cells directly. This technology presents several advantages making it a very useful approach for antibody biology understanding and for antibody use in clinical essays. Among these advantages, single B-cell sorting is characterized by:

• High throughput B-cell sorting – Single B-cell sequencing integrates high throughput platforms for antibody selection which allows to perform different screenings in a highly efficient manner
• Native monoclonal antibody preservation – The main advantage of single B-cell sorting technology is that it preserves the native VH and VL pairing, two domains of monoclonal antibodies critical for antigen recognition and binding. Thus, natural cognate pairing is maintained and development of antibodies with high affinity, stability and specificity is guaranteed.
• Rare antibodies identification – Some antibodies with relevant therapeutic properties are hard to identify. Thanks to the single B-cell screening approach, some rare antibodies are identified directly from B-cells and this limits the risks of unnatural pairing resulting from in vitro screening. Moreover, this approach is ideal for discovery of rare antibodies against challenging targets such as conformational epitopes.
• Antibody diversity maximization – Thanks to the highly sophisticated screening method, single-B cells are screened individually leading to a various repertoire of monoclonal antibodies which increases the B cell diversity.
• Higher efficiency and expedited timelines – Single B-cell screening is a very efficient technology characterized by an unprecedented single-cell-resolution and an exceptional rapid timeline saving months of work as a result of avoiding the cell fusion (hybridoma) and library construction (phage display) steps.