Monoclonal antibody production package details
- Antigen design (design of the most appropriate fragment to be produced)
- Two gene syntheses (each gene optimized for a specific expression system) and 2 subclonings
- Expression tests in two systems taken from E. coli, B. subtilis, P. pastoris, insect cells, and mammalian cells. To be determined by ProteoGenix.
- Production of the antigen in the most appropriate expression system depending on the yield and the project requirements.
- Mice injections (5 mice)
- Immune response control in ELISA
- Selection of the best two mice for two fusions, in order to have a large number and variety of clones
- Screening of the fusion product by ELISA: around 10 x 96-well plates
- Subcloning of 10 parental clones (2-3 rounds of limiting dilutions) examined by ELISA
- Production and purification of 2mg from 10 clones
- Conjugation of all 10 clones to Biotin, HRP or AP
- Test of all the 90 sandwich possibilities
- Level 1 : If we don’t succeed in producing an antibody pair that works in ELISA against the immunogen, we charge EUR 2500
- Level 2 : If we succeed in producing an antibody pair that works in ELISA against the immunogen, we charge a small part of the cost only
- Level 3 : If after testing the purified antibody the customer wants to receive the hybridoma, we charge the full package price
- 10 -30 best clones at parental stage for customer’s selection
- 100 µg of each purified antibody from best pair to confirm hybridoma purchase
- 50 µg of purified protein
- Remaining purified and conjugated antibodies
- 10 hybridoma cell line (four frozen vials)
- 150 µg of purified protein
- Gene in pUC57
- Gene synthesis: 3-4 weeks
- Protein production : 7-9 weeks
- Injections: 8-12 weeks
- Fusion & screening: 5-6 weeks
- Subcloning: 12-18 weeks
- Antibody production: 3-4 weeks
- Conjugation and tests: 2 weeks
- Total: 40-55 weeks
Our application guaranteed monoclonal antibody production packages
Standard ELISA: ATX-PACK1M
Premium ELISA: ATX-PACK2M
Premium WB: ATX-PACK3M
Premium Flow Cytometry: ATX-PACK4M
Premium IF: ATX-PACK6M
Premium IP: ATX-PACK7M
Peptide: ATX-PACK8M
Modification specific: ATX-PACK9M
Premium IHC: ATX-PACK10M