Sandwich ELISA guaranteed monoclonal antibody production

Monoclonal antibody production package details

Antigen design (design of the most appropriate fragment to be produced)
Two gene syntheses (each gene optimized for a specific expression system) and 2 subclonings
Expression tests in two systems taken from E. coli, B. subtilis, P. pastoris, insect cells, and mammalian cells. To be determined by ProteoGenix.
Production of the antigen in the most appropriate expression system depending on the yield and the project requirements.
Mice injections (5 mice)
Immune response control in ELISA
Selection of the best two mice for two fusions, in order to have a large number and variety of clones
Screening of the fusion product by ELISA: around 10 x 96-well plates
Subcloning of 10 parental clones (2-3 rounds of limiting dilutions) examined by ELISA
Production and purification of 2mg from 10 clones
Conjugation of all 10 clones to Biotin, HRP or AP
Test of all the 90 sandwich possibilities

Level 1 : If we don’t succeed in producing an antibody pair that works in ELISA against the immunogen, we charge EUR 2500
Level 2 : If we succeed in producing an antibody pair that works in ELISA against the immunogen, we charge a small part of the cost only
Level 3 : If after testing the purified antibody the customer wants to receive the hybridoma, we charge the full package price

After total payment: