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Nycodenz AG®
XtenCHO ™ Race Starter kit
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Custom services
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Antibody services
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Antigen design
Protein
Peptide
DNA
Custom monoclonal antibody development
Hot
By method
Single B Cell Screening
NEW
Phage display
Hybridoma
By species
Mouse / Rat
Rabbit
Human
Dog
Cat
NEW
Camelids
Pig
NEW
By target
Anti-drug antibodies
Anti-hapten antibodies
By Format
Fab
scFv
VHH
Antibody engineering services
Humanization
Affinity maturation
Antibody-drug conjugates
Bispecific antibodies
CAR-T cell development
Monoclonal antibody characterization
KD determination
Antibody sequencing
Antibody protein sequencing
Recombinant antibody production
Transient antibody expression
Stable cell line generation
Ascites / Culture supernatant
High throughput antibody production
NEW
Polyclonal antibody production
Polyclonal antibody production
Polyclonal Antibody Form
ELISA assay development
Therapeutic antibody services
Therapeutic antibody services
Fully human antibody development services
CAR-T cell development
Custom antibodies against cancer targets
Custom antibodies against viral targets
Protein services
Protein services
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By expression system
E.coli
B. subtilis
Yeast
Baculovirus / insect cells
Mammalian cells
By expression type
Transient expression
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High throughput protein expression
High throughput antibody production
NEW
High throughput protein expression
Protein expression system evaluation and selection
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Gene Synthesis Interactive Form
Online Quote
Gene synthesis services
Peptide services
Peptide services
Custom peptide synthesis
Solid-phase peptide synthesis
Liquid phase peptide synthesis
Peptide Synthesis Interactive Form
Online Quote
Peptide Library
Peptide library generator
HOT
Overlapping peptide libraries
Truncation peptide libraries
Random peptide libraries
Alanine scanning peptide libraries
Scrambled peptide libraries
Positional scanning peptide libraries
Peptide conjugation and modification
Bioreagents
Bioreagents
Antibodies
Antibodies
Primary antibodies
By target
Therapeutic antibodies - Biosimilars
Isotype control antibodies
Anti-viral proteins
Anti-Immune checkpoint
Anti-Biomarker
Nanobodies
Anti-drug antibodies
Tag antibodies
Anti-interferons
Anti-Cytokine
Anti-GPCR
Anti-Chemokines & chemokine receptors
Anti-Spike & Anti-RBD
Anti-Growth factor
Anti-CD
Anti-Enzymes
Anti-TNF
Anti-Interleukins
Anti-Transcription Factors
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WB
FC
IHC
IP
IF
MS
SPR
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Therapeutic Proteins - Biosimilars
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PD1 - PDL1 - PDL2
XtenCHO™
XtenCHO™
XtenCHO™ Race Starter kit
XtenCHO™ Race Transfection kit
XtenCHO™ Race Cells
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XtenCHO ™ Expression medium
XtenCHO ™ Expression medium pack
Cell separation media
Elisa assay kits
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XtenCHO™ Race
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XtenCHO™ Race
Discover the new XtenCHO™ Race Transient Expression System
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Western Blot guaranteed monoclonal antibody production
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Western Blot guaranteed monoclonal antibody production
Monoclonal antibody production package details
Package content
Guarantees and price
Deliverables
Lead time
Antigen design (design of the most appropriate fragment to be produced)
Two gene syntheses (each gene optimized for a specific expression system) and 2 subclonings
Expression tests in two systems taken from
E. coli
,
B. subtilis
,
P. pastoris
, insect cells and mammalian cells. To be determined by ProteoGenix.
Production of the antigen in the most appropriate expression system depending on the yield and the project requirements.
Mice injections (5 mice)
Immune response control in Western Blot (WB)
Selection of the best two mice for two fusions in order to have a large number and variety of clones
Screening of the fusion product directly in Western Blot (WB): around 1000 lanes tested, 42 WB of 24 lanes
Subcloning of two parental clones (2-3 rounds of limiting dilutions) in Western Blot (WB)
Production and purification of 2mg from the best clone
Level 1 : If we don’t succeed in producing an antibody that detects the immunogen in WB, we charge EUR 1 000
Level 2 : If we succeed in producing an antibody that works in WB against the immunogen, we charge a part of the cost
Level 3 : If after testing the purified antibody the customer wants to receive the hybridoma, we charge the full package price
10 -20 best clones at parental stage for customer’s selection
100 µg of purified antibody to confirm hybridoma purchase
50 µg of purified protein
After total payment:
1.9 mg of purified antibody
One hybridoma cell line (four frozen vials)
150 µg of purified protein
Both genes in pUC57
Gene synthesis: 1-2 weeks
Protein production : 3-9 weeks
Injections: 7-11 weeks
Fusion & screening:2-4 weeks
Subcloning: 3-5 weeks
Antibody production:3-4 weeks
Total:19-35 weeks
Get a free quote
Our application guaranteed monoclonal antibody production packages
Standard ELISA: ATX-PACK1M
Premium ELISA: ATX-PACK2M
Premium Flow Cytometry: ATX-PACK4M
Premium sandwich ELISA: ATX-PACK5M
Premium IF: ATX-PACK6M
Premium IP: ATX-PACK7M
Peptide: ATX-PACK8M
Modification specific: ATX-PACK9M
Premium IHC: ATX-PACK10M
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