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Anti-Human MMP13 Monoclonal Antibody (40E09#), on SDS-PAGE. The gel was stained overnight with Coomassie Blue. The purity of the antibody is greater than 95%.
Flow-cytometry using anti-human MMP13 antibody.MDA-MB-231 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human MMP13 antibody monoclonal antibody (Catalog # PTX25216 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # PTX17818) and cells analysed on a NovoCyte Flow Cytometer.
Flow-cytometry using anti-human MMP13 antibody.HeLa cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human MMP13 antibody monoclonal antibody (Catalog # PTX25216 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # PTX17818) and cells analysed on a NovoCyte Flow Cytometer.
Recombinant Human MMP13, N-His(cat. No.ARO-P13754) at 0.5µg/mL (100µL/well) can bind Anti-Human MMP13 Monoclonal Antibody (40E09#) in indirect ELISA with Goat secondary antibody measured by OD450.
ARO-P13754
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