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Human CD34 Monoclonal Antibody(PX-TA1687) was analyzed by size exclusion chromatography with UV detection.
CD34 Transfected CHO cells were stained with an irrelevant antibody (Blue Histogram) or Human CD34 Monoclonal Antibody (ARO-A15896 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using Goat Anti-Mouse IgG Polyclonal Antibody, FITC (PTX17818) and cells analysed on a NovoCyte Flow Cytometer.
KG-1 cells were stained with an irrelevant antibody (Blue Histogram) or Human CD34 Monoclonal Antibody (ARO-A15896 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using Goat Anti-Mouse IgG Polyclonal Antibody, FITC (PTX17818) and cells analysed on a NovoCyte Flow Cytometer.
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