Recombinant Human CFL2 Protein, N-His-SUMO

Description of Recombinant Human CFL2 Protein, N-His-SUMO

Introduction

Recombinant Human CFL2 Protein is a highly versatile and valuable protein that has been widely used in various scientific research and medical applications. It is a type of recombinant protein, meaning it is produced through genetic engineering techniques, specifically through the manipulation of DNA sequences. This allows for the production of large quantities of the protein in a controlled and efficient manner. In this article, we will explore the structure, activity, and applications of this important protein.

Structure of Recombinant Human CFL2 Protein

The recombinant human CFL2 protein is a member of the cofilin family, which are actin-binding proteins involved in regulating the dynamics of actin filaments. It is composed of 166 amino acids and has a molecular weight of approximately 18.6 kDa. The protein contains two actin-binding sites and a phosphorylation site, which are important for its activity.

The structure of recombinant human CFL2 protein has been extensively studied and determined through various techniques, including X-ray crystallography and nuclear magnetic resonance (NMR) spectroscopy. It has been found to have a similar structure to other cofilin family members, with a central alpha-helical domain and a smaller beta-sheet domain. This structure allows the protein to bind to actin filaments and regulate their dynamics.

Activity of Recombinant Human CFL2 Protein

Recombinant human CFL2 protein is primarily involved in regulating the dynamics of actin filaments, which are essential for various cellular processes such as cell motility, cell division, and cell signaling. The protein binds to actin filaments and promotes their disassembly, leading to the turnover and remodeling of the cytoskeleton. This activity is regulated by the phosphorylation of the protein, which can either enhance or inhibit its binding to actin filaments.

In addition to its role in regulating actin dynamics, recombinant human CFL2 protein has also been found to have other functions. It has been shown to interact with other proteins, such as myosin and tropomyosin, and play a role in muscle contraction. It is also involved in cell migration and invasion, making it a potential target for cancer research.

Applications of Recombinant Human CFL2 Protein

The unique structure and activity of recombinant human CFL2 protein make it a valuable tool for various scientific and medical applications. One of its main applications is in the study of actin dynamics and cytoskeleton regulation. Researchers can use the protein to investigate the mechanisms of actin filament disassembly and its role in different cellular processes.

Recombinant human CFL2 protein has also been used in drug discovery and development. Its involvement in cell migration and invasion makes it a potential target for cancer therapeutics. By studying the protein’s activity and interactions, researchers can identify potential drug candidates that can disrupt its function and inhibit cancer cell growth and metastasis.

Furthermore, recombinant human CFL2 protein has been used in the development of diagnostic tools for certain diseases. For example, it has been shown to be a potential biomarker for Alzheimer’s disease, as its levels have been found to be altered in the brains of patients with this condition. This opens up the possibility of using the protein as a diagnostic tool for early detection and monitoring of the disease.

Conclusion

In summary, recombinant human CFL2 protein is a highly valuable and versatile protein with a unique structure and activity. Its involvement in regulating actin dynamics and other cellular processes makes it a crucial tool for scientific research and medical applications. With ongoing studies and advancements in genetic engineering techniques, the potential uses and benefits of this protein are bound to expand in the future.