Recombinant Human HMOX2, N-His

Description of Recombinant Human HMOX2, N-His

Introduction

Recombinant Human HMOX2, N-His is a protein that plays a crucial role in the body’s defense against oxidative stress and inflammation. This protein is produced through recombinant DNA technology and is used for various research and therapeutic purposes. In this article, we will discuss the structure, activity, and applications of Recombinant Human HMOX2, N-His as a potential drug target.

Structure of Recombinant Human HMOX2, N-His

Recombinant Human HMOX2, N-His is a 32 kDa protein that belongs to the heme oxygenase family. It is composed of 288 amino acids and has a conserved heme-binding domain. This protein has a similar structure to its isoform HMOX1, with 42% sequence identity. It also contains a N-terminal His-tag, which is essential for purification and detection purposes.

Activity of this protein

The main function of Recombinant Human HMOX2, N-His is to catalyze the degradation of heme into biliverdin, carbon monoxide, and iron. This process, also known as heme catabolism, is essential for maintaining cellular homeostasis. The breakdown of heme by HMOX2, N-His produces biliverdin, which is further converted into bilirubin, a potent antioxidant. Carbon monoxide, on the other hand, acts as a signaling molecule and helps in regulating various physiological processes, such as blood pressure and inflammation. The release of iron from heme also plays a crucial role in iron recycling and maintaining iron balance in the body.

Role as a Drug Target

The activity of HMOX2, N-His in heme catabolism makes it a potential drug target for various diseases. Oxidative stress and inflammation are major contributors to the development of several diseases, including cancer, cardiovascular diseases, and neurodegenerative disorders. HMOX2, N-His, by producing bilirubin and carbon monoxide, can help in reducing oxidative stress and inflammation and thus, can be targeted for therapeutic purposes.

Cancer

The overexpression of HMOX2, N-His has been observed in various types of cancer, including breast, colorectal, and lung cancer. This overexpression has been linked to increased cell proliferation and resistance to chemotherapy. Targeting HMOX2, N-His can help in reducing cell proliferation and sensitizing cancer cells to chemotherapy drugs.

Cardiovascular Diseases

HMOX2, N-His has been shown to play a protective role in cardiovascular diseases. Its ability to produce carbon monoxide, a vasodilator, helps in lowering blood pressure and improving blood flow. In addition, its antioxidant activity can help in preventing the development of atherosclerosis, a major risk factor for cardiovascular diseases.

Neurodegenerative Disorders

Oxidative stress and inflammation are major contributors to the development of neurodegenerative disorders, such as Alzheimer’s and Parkinson’s disease. HMOX2, N-His, by producing bilirubin and carbon monoxide, can help in reducing oxidative stress and inflammation in the brain, thus providing a potential therapeutic target for these diseases.

Applications of Recombinant Human HMOX2, N-His

Recombinant Human HMOX2, N-His has various applications in research and therapeutic settings. Its ability to produce bilirubin and carbon monoxide makes it a valuable tool for studying the role of these molecules in different diseases. In addition, its role in heme catabolism makes it a potential target for developing new drugs for various diseases.