Recombinant Human IL1RAP, N-His

Introduction to Recombinant Human IL1RAP

Recombinant Human IL1RAP (Interleukin-1 receptor accessory protein) is a protein that plays a crucial role in the immune response and inflammatory processes. It is a member of the interleukin-1 receptor family and is encoded by the IL1RAP gene. This protein is widely used in scientific research and has potential therapeutic applications in various diseases.

Structure of Recombinant Human IL1RAP

Recombinant Human IL1RAP is a 55 kDa transmembrane protein that consists of 447 amino acids. It contains a signal peptide, an extracellular domain, a transmembrane domain, and a cytoplasmic domain. The extracellular domain is responsible for binding to its ligands, IL-1α and IL-1β, while the cytoplasmic domain is involved in signal transduction. The crystal structure of recombinant human IL1RAP has been determined, revealing a unique fold with a central β-sheet surrounded by α-helices.

Activity of Recombinant Human IL1RAP

Recombinant Human IL1RAP is a co-receptor for the pro-inflammatory cytokines IL-1α and IL-1β. It is essential for the activation of the IL-1 signaling pathway, which plays a crucial role in the immune response and inflammation. The binding of IL-1α or IL-1β to IL1RAP results in the recruitment of the IL-1 receptor type I (IL-1R1), leading to the formation of a signaling complex. This complex then activates downstream signaling pathways, including NF-κB and MAPK, which regulate the expression of pro-inflammatory genes. IL1RAP also interacts with other receptors, such as IL-1R3 and IL-1R8, to modulate the activity of IL-1 signaling.

Application of Recombinant Human IL1RAP

Recombinant Human IL1RAP has various applications in scientific research, including the study of immune response and inflammation. It is also used in the development of therapeutic strategies for diseases associated with dysregulated IL-1 signaling, such as rheumatoid arthritis, inflammatory bowel disease, and sepsis. Recombinant human IL1RAP has been shown to have therapeutic potential in preclinical studies, with promising results in animal models of inflammatory diseases.

Recombinant Human IL1RAP as a recombinant protein

Recombinant Human IL1RAP is produced using recombinant DNA technology, where the IL1RAP gene is inserted into a suitable expression vector and then expressed in a host cell. This results in the production of a pure and biologically active form of IL1RAP that can be used in various research applications. Recombinant human IL1RAP is available from various commercial sources and is widely used in studies investigating the role of IL1RAP in different diseases.

Recombinant Human IL1RAP as an antigen

Recombinant Human IL1RAP can also be used as an antigen in immunological assays, such as ELISA and Western blot, to detect the presence of IL1RAP in biological samples. It is also used in the development of antibodies against IL1RAP for research and diagnostic purposes. The availability of recombinant human IL1RAP has greatly facilitated the study of IL1RAP and its role in various diseases.

Conclusion

Recombinant Human IL1RAP is a crucial protein involved in the immune response and inflammation. Its structure, activity, and applications have been extensively studied, and it has shown potential as a therapeutic target for various diseases. The availability of recombinant human IL1RAP has greatly advanced our understanding of IL1RAP biology and its role in disease pathogenesis. Further research on this protein may lead to the development of novel treatments for inflammatory diseases.</