Recombinant Human LSR, N-His

Description of Recombinant Human LSR, N-His

Introduction

Recombinant Human LSR (leukocyte-specific transcript 1) is a type I transmembrane protein that plays a crucial role in cell adhesion and signaling. It is a member of the immunoglobulin superfamily and is expressed on the surface of various cell types, including leukocytes, endothelial cells, and neurons. This article will provide a comprehensive overview of the structure, activity, and applications of Recombinant Human LSR.

Structure of Recombinant Human LSR

Recombinant Human LSR is a 105 kDa protein composed of 933 amino acids. It consists of an extracellular domain, a transmembrane domain, and a cytoplasmic tail. The extracellular domain contains two immunoglobulin-like domains, IgV and IgC2, which are responsible for the protein’s adhesive properties. The transmembrane domain anchors the protein to the cell membrane, while the cytoplasmic tail contains signaling motifs that interact with intracellular proteins.

Activity of Recombinant Human LSR

The primary function of Recombinant Human LSR is to mediate cell-cell adhesion. It does so by binding to other LSR molecules on adjacent cells, as well as to other proteins such as integrins and cadherins. This adhesive activity is crucial for various biological processes, including immune response, angiogenesis, and neuronal development.

In addition to its adhesive properties, Recombinant Human LSR also has signaling functions. It can interact with intracellular proteins such as PDZ domain-containing proteins, which regulate cell signaling pathways. This interaction can modulate various cellular processes, including cell proliferation, differentiation, and survival.

Applications of Recombinant Human LSR

Recombinant Protein Production

Recombinant Human LSR is widely used in the production of recombinant proteins. Its adhesive properties make it an ideal fusion partner for other proteins, allowing for efficient purification of the recombinant protein. This is particularly useful in the production of difficult-to-express proteins, as the presence of LSR can improve their solubility and stability.

Antigen for Antibody Production

Recombinant Human LSR is also used as an antigen for antibody production. Its high expression on the surface of various cell types makes it an attractive target for generating specific antibodies. These antibodies can be used for various applications, including immunohistochemistry, immunofluorescence, and flow cytometry.

Research Tool

Recombinant Human LSR is a valuable research tool for studying cell adhesion and signaling. Its adhesive and signaling properties make it a useful tool for investigating the mechanisms of these processes. Additionally, recombinant LSR can be used to modulate cell adhesion and signaling in vitro, allowing for the study of their effects on cellular behavior.

Therapeutic Applications

Given its role in various biological processes, Recombinant Human LSR has potential therapeutic applications. It has been implicated in diseases such as cancer and inflammatory disorders, making it a potential target for drug development. Additionally, its adhesive properties make it a promising candidate for the development of targeted drug delivery systems.

Conclusion

In summary, Recombinant Human LSR is a multifunctional protein with important roles in cell adhesion and signaling. Its structure, activity, and applications make it a valuable tool in protein production, antibody generation, research, and potential therapeutics. Further studies on this protein will continue to enhance our understanding of its functions and potential applications.