Recombinant Human MDA5/IFIH1 Protein, N-His

Description of Recombinant Human MDA5/IFIH1 Protein, N-His

Introduction

Recombinant Human MDA5/IFIH1 Protein, also known as Melanoma Differentiation-Associated Protein 5 or Interferon-Induced with Helicase C Domain 1, is a vital component of the innate immune system. This protein plays a crucial role in the detection and response to viral infections, making it a valuable tool in the field of immunology and infectious diseases.

Structure of Recombinant Human MDA5/IFIH1 Protein

The MDA5/IFIH1 protein is a 1018 amino acid long protein with a molecular weight of approximately 114 kDa. It contains two N-terminal caspase activation and recruitment domains (CARDs) and a C-terminal DExD/H-box helicase domain. The CARD domains are involved in protein-protein interactions, while the helicase domain is responsible for the recognition and binding of viral RNA.

The recombinant form of MDA5/IFIH1 protein is produced in a bacterial expression system using genetic engineering techniques. This results in a highly pure and bioactive protein that is free from contaminants and endotoxins.

Activity of Recombinant Human MDA5/IFIH1 Protein

The main function of MDA5/IFIH1 protein is to detect viral RNA and initiate an immune response. Upon binding to viral RNA, the CARD domains of MDA5/IFIH1 interact with the CARD domains of the adaptor protein MAVS, leading to the activation of the transcription factors NF-kB and IRF3. These transcription factors then induce the expression of type I interferons and other pro-inflammatory cytokines, which play a crucial role in fighting viral infections.

In addition to its role in antiviral immunity, MDA5/IFIH1 protein has also been shown to play a role in the recognition and clearance of intracellular bacteria. Studies have also suggested a potential role for MDA5/IFIH1 in autoimmune diseases, as mutations in the gene encoding this protein have been linked to autoimmune disorders such as type 1 diabetes and systemic lupus erythematosus.

Applications of Recombinant Human MDA5/IFIH1 Protein

Recombinant Human MDA5/IFIH1 Protein has a wide range of applications in both research and clinical settings. Its ability to detect and respond to viral infections makes it a valuable tool for studying viral pathogenesis and developing antiviral therapies. The recombinant protein can also be used to screen for potential antiviral drugs and to study the mechanisms of viral evasion of the immune system.

In the clinic, MDA5/IFIH1 protein can be used as a diagnostic tool for viral infections. Its high specificity and sensitivity make it a reliable biomarker for the detection of various viral diseases. In addition, the recombinant protein can also be used as a therapeutic agent for the treatment of viral infections. By activating the immune system, MDA5/IFIH1 protein can help in the clearance of viral infections and reduce the severity of symptoms.

Conclusion

Recombinant Human MDA5/IFIH1 Protein is a crucial component of the innate immune system with a vital role in the detection and response to viral infections. Its structure, activity, and applications make it a valuable tool in the field of immunology and infectious diseases. With ongoing research and advancements in technology, the potential of this protein in both research and clinical settings is continuously expanding.

Keywords: recombinant protein, antigen, MDA5/IFIH1, innate immunity, viral infections, immunology, infectious diseases.