Recombinant Human NKX2-5 Protein, N-GST & C-His

Description of Recombinant Human NKX2-5 Protein, N-GST & C-His

Introduction to Recombinant Human NKX2-5 Protein

Recombinant Human NKX2-5 Protein is a synthetic form of the NKX2-5 protein, which is a transcription factor that plays a crucial role in cardiac development and function. This recombinant protein is produced through genetic engineering techniques, where the gene for NKX2-5 is inserted into a host organism, such as bacteria or yeast, to produce large quantities of the protein for research and therapeutic purposes.

Structure of Recombinant Human NKX2-5 Protein

The NKX2-5 protein belongs to the NK-2 family of homeodomain-containing transcription factors. It is composed of 324 amino acids and has a molecular weight of approximately 36 kDa. The recombinant form of this protein is identical to the native form, with the only difference being the absence of post-translational modifications that occur in the human body. This makes it a highly pure and stable protein for use in various scientific applications.

The structure of NKX2-5 protein consists of three domains – the N-terminal domain, the homeodomain, and the C-terminal domain. The N-terminal domain is responsible for protein-protein interactions, while the homeodomain binds to specific DNA sequences to regulate gene expression. The C-terminal domain is involved in transcriptional activation and can also interact with other transcription factors to modulate gene expression.

Activity of Recombinant Human NKX2-5 Protein

The main function of NKX2-5 protein is to regulate the expression of genes involved in cardiac development and function. It is essential for the formation of the heart during embryonic development and also plays a role in maintaining the proper functioning of the heart in adults. NKX2-5 protein has been shown to regulate the expression of genes involved in cardiac muscle contraction, cell proliferation, and differentiation.

Studies have also shown that mutations in the NKX2-5 gene can lead to congenital heart defects, highlighting the importance of this protein in cardiac development. The recombinant form of NKX2-5 protein can be used to study the molecular mechanisms underlying these defects and to develop potential therapeutic interventions.

Applications of Recombinant Human NKX2-5 Protein

Recombinant Human NKX2-5 Protein has a wide range of applications in the field of cardiovascular research. It can be used in in vitro and in vivo studies to investigate the role of NKX2-5 in cardiac development and function. The recombinant protein can also be used to screen for potential drugs that can target NKX2-5 and its downstream pathways for the treatment of heart diseases.

Furthermore, the high purity and stability of recombinant NKX2-5 protein make it an ideal antigen for the development of diagnostic assays for heart diseases. It can also be used as a standard in immunoassays to measure the levels of NKX2-5 in patient samples, providing valuable information for disease diagnosis and prognosis.

In addition to its research and diagnostic applications, recombinant NKX2-5 protein also has therapeutic potential. It can be used in gene therapy to deliver functional NKX2-5 gene to patients with congenital heart defects caused by mutations in the NKX2-5 gene. This approach has shown promising results in preclinical studies and holds great promise for the treatment of these conditions in the future.

Conclusion

Recombinant Human NKX2-5 Protein is a valuable tool in the study of cardiac development and function. Its well-defined structure, activity, and various applications make it an essential protein for researchers and clinicians alike. With ongoing research and advancements in genetic engineering techniques, the potential of recombinant NKX2-5 protein in the field of cardiovascular research and therapy is vast and promising.