Recombinant Human PCBP2 Protein, N-His

Description of Recombinant Human PCBP2 Protein, N-His

Introduction

Recombinant Human PCBP2 Protein, also known as poly(rC)-binding protein 2, is a highly conserved RNA-binding protein that plays a crucial role in various cellular processes. It is encoded by the PCBP2 gene and is found in all eukaryotic organisms. In this article, we will discuss the structure, activity, and application of Recombinant Human PCBP2 Protein.

Structure of Recombinant Human PCBP2 Protein

Recombinant Human PCBP2 Protein is a 356 amino acid protein with a molecular weight of approximately 40 kDa. It consists of three RNA recognition motifs (RRMs) that are responsible for its RNA-binding activity. These RRMs are arranged in tandem, with RRM1 and RRM2 being highly conserved, while RRM3 has a unique sequence. The protein also contains a proline-rich region that is involved in protein-protein interactions.

Activity of Recombinant Human PCBP2 Protein

Recombinant Human PCBP2 Protein is a multifunctional protein that has been shown to interact with various cellular components, including RNA, DNA, and proteins. Its main function is to bind to single-stranded poly(C) sequences in RNA and regulate gene expression at the post-transcriptional level. It does so by either stabilizing or destabilizing the target RNA, depending on the specific sequence and context.

In addition to its role in RNA binding, Recombinant Human PCBP2 Protein has been implicated in other cellular processes, such as DNA replication, translation, and splicing. It has also been shown to interact with various proteins involved in these processes, further highlighting its multifunctional nature.

Application of Recombinant Human PCBP2 Protein

Recombinant Human PCBP2 Protein has a wide range of applications in both basic and applied research. Its ability to bind to RNA with high specificity and affinity makes it a valuable tool for studying post-transcriptional gene regulation. It can be used in techniques such as RNA pull-down assays and RNA immunoprecipitation to identify and characterize target RNAs.

Moreover, Recombinant Human PCBP2 Protein has been shown to play a role in viral infections, making it a potential target for antiviral therapies. For example, it has been shown to interact with the hepatitis C virus (HCV) RNA and regulate its translation, making it a potential target for the treatment of HCV infections.

Furthermore, Recombinant Human PCBP2 Protein has been shown to be dysregulated in various cancers, including breast, lung, and colon cancer. This dysregulation has been linked to its role in regulating the expression of oncogenes and tumor suppressor genes. Thus, it has potential as a biomarker for cancer diagnosis and a target for cancer therapy.

Conclusion

In summary, Recombinant Human PCBP2 Protein is a highly conserved RNA-binding protein with a multifunctional role in various cellular processes. Its structure, activity, and application make it a valuable tool for studying post-transcriptional gene regulation, as well as a potential target for antiviral and cancer therapies. Further research on this protein will likely uncover more of its functions and potential applications in the future.