Recombinant Human POLB Protein, N-His

Description of Recombinant Human POLB Protein, N-His

Introduction

Recombinant Human POLB Protein, also known as DNA Polymerase Beta, is a highly conserved enzyme that plays a crucial role in DNA repair and replication processes. This protein is produced through recombinant DNA technology, making it a valuable tool in various scientific and medical applications. In this article, we will discuss the structure, activity, and applications of Recombinant Human POLB Protein.

Structure of Recombinant Human POLB Protein

Recombinant Human POLB Protein is a 39 kDa protein consisting of 335 amino acids. It is composed of a catalytic domain and a C-terminal domain, which are connected by a flexible linker region. The catalytic domain contains the active site, where the polymerase activity takes place, while the C-terminal domain is responsible for the protein’s interaction with other DNA repair proteins.

The crystal structure of Recombinant Human POLB Protein has been extensively studied, revealing its unique features. It has a compact structure with a thumb, palm, and fingers subdomains, which are characteristic of DNA polymerases. The active site is located in the palm subdomain and contains two divalent metal ions, which are essential for the polymerase activity.

Activity of Recombinant Human POLB Protein

Recombinant Human POLB Protein is a DNA polymerase that catalyzes the addition of nucleotides to the 3′ end of a DNA strand during DNA repair and replication processes. It has a high fidelity in incorporating the correct nucleotide, making it crucial for maintaining the integrity of the genome.

One of the unique features of Recombinant Human POLB Protein is its ability to perform both polymerase and 5′-deoxyribose-5-phosphate (dRP) lyase activities. The dRP lyase activity removes the damaged nucleotide from the DNA strand, allowing the polymerase activity to insert the correct nucleotide. This dual function makes Recombinant Human POLB Protein an essential enzyme in base excision repair, which is the primary mechanism for repairing DNA damage caused by oxidative stress.

Applications of Recombinant Human POLB Protein

Recombinant Human POLB Protein has various applications in both research and medical fields. Its high fidelity and ability to perform both polymerase and dRP lyase activities make it a valuable tool in studying DNA repair mechanisms and their role in diseases such as cancer.

In research, Recombinant Human POLB Protein is used for in vitro studies to understand its role in DNA repair and replication processes. It is also used in structural biology studies to investigate the interactions between Recombinant Human POLB Protein and other DNA repair proteins.

In the medical field, Recombinant Human POLB Protein has potential applications in cancer therapy. As cancer cells have a high level of oxidative stress, they heavily rely on the base excision repair mechanism. Inhibiting Recombinant Human POLB Protein’s activity could selectively kill cancer cells, making it a promising target for cancer treatment.

Furthermore, Recombinant Human POLB Protein can be used in diagnostic assays for detecting DNA damage and monitoring the effectiveness of cancer treatments. Its high specificity and sensitivity make it an ideal candidate for developing diagnostic tools.

Conclusion

In summary, Recombinant Human POLB Protein is a crucial enzyme in DNA repair and replication processes. Its unique structure and dual activities make it an essential tool in scientific research and have potential applications in cancer therapy and diagnostics. With further studies and advancements in recombinant DNA technology, Recombinant Human POLB Protein could have even more significant impacts in various fields.