Recombinant Human PTPRN, N-His

Introduction to Recombinant Human PTPRN

Recombinant Human PTPRN, also known as protein tyrosine phosphatase receptor type N, is a recombinant protein that has been produced through genetic engineering techniques. This protein plays a crucial role in regulating cellular signaling pathways by dephosphorylating tyrosine residues on target proteins. It is a member of the protein tyrosine phosphatase (PTP) family and is highly expressed in the brain, pancreas, and liver.

Structure of Recombinant Human PTPRN

Recombinant Human PTPRN is a 923 amino acid protein with a molecular weight of approximately 105 kDa. It consists of a single catalytic domain, a transmembrane domain, and a cytoplasmic domain. The catalytic domain contains a conserved cysteine residue that is essential for its phosphatase activity. The transmembrane domain anchors the protein to the cell membrane, while the cytoplasmic domain contains regulatory motifs that control its activity.

Activity of Recombinant Human PTPRN

Recombinant Human PTPRN is a phosphatase enzyme that specifically dephosphorylates tyrosine residues on target proteins. It plays a crucial role in regulating various cellular processes such as cell growth, differentiation, and survival. This protein is involved in the regulation of insulin signaling, which is essential for maintaining glucose homeostasis. It also plays a role in neuronal development and synaptic plasticity, making it a potential target for neurological disorders.

The activity of Recombinant Human PTPRN is tightly regulated by various mechanisms. It can be activated by binding to its ligand, pleiotrophin, or through phosphorylation of its regulatory motifs. On the other hand, it can be inhibited by interacting with other proteins or through oxidation of its catalytic cysteine residue.

Application of Recombinant Human PTPRN

Recombinant Human PTPRN has various applications in both research and therapeutic fields. Its role in regulating insulin signaling makes it a potential target for the treatment of diabetes. In addition, its involvement in neurological disorders such as Alzheimer’s disease and Parkinson’s disease makes it a potential therapeutic target for these conditions.

In research, Recombinant Human PTPRN is widely used as a tool to study cellular signaling pathways. Its phosphatase activity allows researchers to investigate the role of tyrosine phosphorylation in various cellular processes. It is also used in drug discovery and development, as it can be used to screen for potential inhibitors of its activity.

Furthermore, Recombinant Human PTPRN is also used in diagnostic assays for various diseases. Its expression levels have been found to be altered in certain cancers, making it a potential biomarker for cancer diagnosis and prognosis.

Conclusion

Recombinant Human PTPRN is a crucial protein involved in regulating cellular signaling pathways. Its structure, activity, and applications make it a valuable tool for both research and therapeutic purposes. Further studies on this protein may lead to the development of novel treatments for various diseases, making it an important area of research in the field of biotechnology.