Recombinant Mouse ADGRE1/EMR1 Protein, N-His

Description of Recombinant Mouse ADGRE1/EMR1 Protein, N-His

Recombinant Mouse ADGRE1/EMR1 Protein: Structure, Activity, and Applications

Introduction

Recombinant proteins are proteins that are produced in a laboratory setting through genetic engineering techniques. These proteins are used in various scientific and medical applications, including research, diagnostics, and therapeutics. One such recombinant protein is the mouse ADGRE1/EMR1 protein, which has gained significant attention in recent years due to its diverse structure, activity, and potential applications.

Structure of Recombinant Mouse ADGRE1/EMR1 Protein

The ADGRE1/EMR1 protein, also known as F4/80, is a transmembrane protein that belongs to the EGF-TM7 family of proteins. It is primarily expressed on the surface of macrophages, a type of immune cell, and is involved in various cellular processes, including cell adhesion, migration, and phagocytosis. The recombinant mouse ADGRE1/EMR1 protein is produced by inserting the gene encoding for this protein into a suitable expression vector and then expressing it in a host cell, such as E. coli or mammalian cells.

The recombinant protein has a similar structure to its native form, with an extracellular domain consisting of seven EGF-like domains and a single transmembrane domain. The extracellular domain is responsible for the protein’s interaction with its ligands, such as L1 and galectin-3, while the transmembrane domain anchors the protein to the cell membrane.

Activity of Recombinant Mouse ADGRE1/EMR1 Protein

The ADGRE1/EMR1 protein is a multifunctional protein that plays a crucial role in the immune response. It is involved in the recognition and clearance of pathogens, as well as the regulation of inflammation. The recombinant form of this protein has been extensively studied, and its activity has been shown to be similar to its native form.

One of the key activities of the recombinant mouse ADGRE1/EMR1 protein is its role in phagocytosis, the process by which immune cells engulf and destroy foreign particles. This protein has been shown to enhance the phagocytic activity of macrophages, making it a valuable tool for studying the immune response to various pathogens.

In addition to phagocytosis, the recombinant protein has also been shown to regulate cell adhesion and migration. It can interact with other proteins on the cell surface, such as integrins, to facilitate cell movement and communication. This activity is crucial for the proper functioning of immune cells and has potential applications in tissue repair and regeneration.

Applications of Recombinant Mouse ADGRE1/EMR1 Protein

The recombinant mouse ADGRE1/EMR1 protein has a wide range of potential applications in various fields of research and medicine. Its ability to modulate immune responses makes it a valuable tool for studying immune-related diseases and developing new therapeutics.

One potential application of this protein is in cancer research. The ADGRE1/EMR1 protein has been shown to be overexpressed in certain types of cancer, and its role in cell adhesion and migration suggests that it may play a role in tumor metastasis. The recombinant protein can be used to study the mechanisms of cancer progression and potentially develop new treatments targeting this protein.

Another potential application is in the development of vaccines. The ADGRE1/EMR1 protein has been shown to be involved in the immune response to certain pathogens, making it a potential target for vaccine development. The recombinant protein can be used to study the immune response to these pathogens and potentially aid in the development of more effective vaccines.

In addition to research applications, the recombinant protein also has potential diagnostic and therapeutic applications. Its ability to target and regulate immune cells makes it a promising candidate for immunotherapy, a type of treatment that uses the body’s immune system to fight diseases. It can also be used as a diagnostic tool to detect