Recombinant Mouse CD200 Protein, N-His

Description of Recombinant Mouse CD200 Protein, N-His

Introduction to Recombinant Mouse CD200 Protein

Recombinant Mouse CD200 Protein, also known as OX-2 glycoprotein, is a type I membrane protein that belongs to the immunoglobulin superfamily. It is encoded by the Cd200 gene and is expressed on various cell types, including neurons, endothelial cells, and immune cells. CD200 is a highly conserved protein, with human and mouse CD200 sharing 73% amino acid sequence identity.

Structure of Recombinant Mouse CD200 Protein

The recombinant mouse CD200 protein is a 279 amino acid protein with a molecular weight of approximately 30 kDa. It contains a single immunoglobulin (Ig)-like domain in its extracellular region, followed by a transmembrane domain and a short cytoplasmic tail. The Ig-like domain is responsible for the interaction with its receptor, CD200R, which is expressed on myeloid cells and plays a crucial role in regulating immune responses.

The crystal structure of mouse CD200 has been determined, revealing a horseshoe-shaped molecule with a conserved disulfide bond between two cysteine residues. This structure is similar to other members of the immunoglobulin superfamily, such as CD28 and CTLA-4, which also have important roles in immune regulation.

Activity of Recombinant Mouse CD200 Protein

The main function of CD200 is to regulate immune responses by interacting with its receptor, CD200R. This interaction leads to the activation of inhibitory signaling pathways, resulting in the suppression of immune cell activation and cytokine production. This mechanism is essential for maintaining immune homeostasis and preventing excessive inflammation.

CD200 has also been shown to have neuroprotective effects. It is expressed on neurons and has been found to protect against neuronal damage in models of stroke, Alzheimer’s disease, and multiple sclerosis. This neuroprotective function is thought to be mediated by the interaction of CD200 with CD200R expressed on microglia, the immune cells of the central nervous system.

Application of Recombinant Mouse CD200 Protein

The recombinant mouse CD200 protein has been widely used in research studies to investigate its role in immune regulation and neuroprotection. It has also been used in preclinical studies to evaluate its potential as a therapeutic target for various diseases.

One potential application of recombinant mouse CD200 protein is in the treatment of autoimmune diseases. In multiple sclerosis, for example, the CD200-CD200R pathway has been shown to play a critical role in regulating the inflammatory response. Therefore, targeting this pathway with recombinant CD200 protein could potentially provide a new approach for treating this disease.

Another potential application is in the field of cancer immunotherapy. CD200 has been found to be overexpressed in various types of cancer, including leukemia, lymphoma, and solid tumors. This overexpression has been linked to immune evasion and tumor progression. Therefore, targeting CD200 with recombinant protein could potentially enhance anti-tumor immune responses and improve the efficacy of cancer treatments.

Conclusion

In summary, recombinant mouse CD200 protein is a key regulator of immune responses and neuroprotection. Its structure, activity, and potential applications make it a valuable tool for research and a promising therapeutic target for various diseases. Further studies on the function of CD200 and its interaction with CD200R will provide deeper insights into its role in immune regulation and potential therapeutic applications.