Recombinant Mouse QSOX1 Protein, N-His

Description of Recombinant Mouse QSOX1 Protein, N-His

Introduction

Recombinant Mouse QSOX1 Protein, also known as Quiescin Sulfhydryl Oxidase 1, is a highly conserved enzyme that plays a crucial role in protein folding and disulfide bond formation. This protein is encoded by the QSOX1 gene and is found in various tissues and cell types in mice.

Structure of Recombinant Mouse QSOX1 Protein

The recombinant form of QSOX1 is a 70 kDa protein consisting of 614 amino acids. It contains a signal peptide, a thioredoxin-like domain, and two active sites with highly conserved CXXC motifs. The signal peptide is responsible for directing the protein to the endoplasmic reticulum, where it undergoes post-translational modifications before being secreted into the extracellular space.

The thioredoxin-like domain of QSOX1 is responsible for its oxidase activity, which is essential for the formation of disulfide bonds in proteins. The two active sites, located in the C-terminal region of the protein, contain the conserved CXXC motifs that act as redox centers and facilitate the transfer of electrons during the oxidation process.

Activity of Recombinant Mouse QSOX1 Protein

The main function of QSOX1 is to catalyze the oxidation of sulfhydryl groups in proteins, which is a crucial step in the folding and stabilization of newly synthesized proteins. This process is essential for the proper functioning of many secreted and membrane-bound proteins, including enzymes, hormones, and receptors.

In addition to its oxidase activity, QSOX1 also has disulfide reductase activity, which allows it to reduce disulfide bonds in misfolded proteins and facilitate their degradation. This activity is crucial for maintaining protein homeostasis and preventing the accumulation of misfolded proteins, which can lead to cellular dysfunction and disease.

Furthermore, QSOX1 has been shown to play a role in the maturation of extracellular matrix proteins, such as collagen and fibronectin. It is also involved in the formation of disulfide bonds in immunoglobulins, which are important components of the immune system.

Application of Recombinant Mouse QSOX1 Protein

The recombinant form of QSOX1 has been widely used in various research applications, including protein folding studies, protein engineering, and drug discovery. It is also used in the production of recombinant proteins with correct disulfide bond formation, which is essential for their stability and activity.

Moreover, QSOX1 has been implicated in several diseases, including cancer, neurodegenerative disorders, and cardiovascular diseases. As such, it has become a potential therapeutic target, and recombinant QSOX1 protein is being studied for its potential use in the treatment of these diseases.

Additionally, QSOX1 has been identified as a potential biomarker for certain types of cancer, making it a valuable tool for cancer diagnosis and prognosis. Recombinant QSOX1 protein can be used in diagnostic tests to detect the presence of QSOX1 in patient samples, which can aid in the early detection and treatment of cancer.

Conclusion

In summary, Recombinant Mouse QSOX1 Protein is a highly conserved enzyme that plays a critical role in protein folding and disulfide bond formation. Its structure, activity, and applications make it a valuable tool in various research areas, including protein biochemistry, drug discovery, and disease diagnosis and treatment. Further studies on QSOX1 and its recombinant form may provide valuable insights into its role in health and disease, and potentially lead to the development of new therapeutic strategies.