Recombinant Yersinia enterocolitica YopH Protein, N-His

Description of Recombinant Yersinia enterocolitica YopH Protein, N-His

Title: Introduction to Recombinant Yersinia enterocolitica YopH Protein

Recombinant Yersinia enterocolitica YopH protein, also known as Yersinia outer protein H, is a bacterial protein that is produced by the pathogen Yersinia enterocolitica. This protein plays a crucial role in the virulence and pathogenicity of the bacteria, making it an important target for research and potential therapeutic applications.

Structure of Recombinant Yersinia enterocolitica YopH Protein

The YopH protein is a type III secretion system (T3SS) effector protein, meaning it is secreted by the bacteria into the host cell during infection. It is a 72-kDa protein consisting of 627 amino acids and is highly conserved among different strains of Yersinia enterocolitica. The protein has a unique structure, with a central catalytic domain flanked by two domains, the N-terminal domain and the C-terminal domain. The catalytic domain contains the active site, which is responsible for the protein’s phosphatase activity.

Activity of Recombinant Yersinia enterocolitica YopH Protein

The YopH protein is a potent phosphatase that targets and dephosphorylates several host cell proteins involved in the immune response, such as focal adhesion kinase (FAK) and paxillin. By dephosphorylating these proteins, YopH interferes with the host cell’s signaling pathways, leading to disruption of the cytoskeleton and inhibition of the host cell’s ability to mount an effective immune response. This allows the bacteria to evade the host’s immune system and establish infection.

Application of Recombinant Yersinia enterocolitica YopH Protein

Recombinant YopH protein has been extensively studied for its potential applications in both research and medicine. One of the major applications of this protein is in the development of vaccines against Yersinia enterocolitica infections. The YopH protein is a major virulence factor of the bacteria, and targeting it with a vaccine can potentially prevent or reduce the severity of infections.

In addition to its role in vaccine development, recombinant YopH protein has also been studied for its potential as a therapeutic target. Inhibition of YopH activity has been shown to reduce the virulence of Yersinia enterocolitica and other related pathogens, making it a promising target for the development of new antimicrobial drugs.

Furthermore, recombinant YopH protein has also been used in research to study the mechanisms of bacterial pathogenesis and the host immune response. By understanding how YopH interacts with host cell proteins, researchers can gain insights into the complex interactions between bacteria and their hosts, leading to the development of new strategies for treating and preventing infections.

Conclusion

In summary, recombinant Yersinia enterocolitica YopH protein is a key virulence factor of the pathogenic bacteria Yersinia enterocolitica. Its unique structure and potent phosphatase activity make it an important target for research and potential therapeutic applications. Further studies on this protein can lead to the development of effective vaccines and treatments against Yersinia enterocolitica infections, as well as a better understanding of bacterial pathogenesis and host immune response.