The BasoFlowEx Kit is intended for flow cytometry examination of IgE-mediated allergic reactions via analysis of CD63 antigen exposition on basophils in human heparinized whole blood upon allergen stimulation.
Commercially available allergens can be used for the stimulation.
Kit Content:
ED7043-1 Stimulation Buffer – 5 lyophilized vials, 1 vial is intended for stimulation of 20 tubes.
ED7043-2 Stimulation Control – 2 lyophilized vials, 1 vial is intended for stimulation of 25 positive controls.
ED7043-3 Staining Reagent – 1 vial containing 2 ml of premixed antibody cocktail: anti-CD63, FITC labeled + anti-CD203c, PE labeled.
ED7043-4 Lysing Solution – 30 ml.
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