Recombinant Human ADCY9, N-His

Description of Recombinant Human ADCY9, N-His

Introduction to Recombinant Human ADCY9, N-His

Recombinant Human ADCY9, N-His is a protein that has been engineered through recombinant DNA technology to mimic the human ADCY9 protein. It is also known as human adenylate cyclase 9 and is a member of the adenylate cyclase family of enzymes. This protein plays a crucial role in the regulation of cellular signaling pathways and is a potential drug target for various diseases. In this article, we will discuss the structure, activity, and application of Recombinant Human ADCY9, N-His.

Structure of Recombinant Human ADCY9, N-His

Recombinant Human ADCY9, N-His has a similar structure to the native human ADCY9 protein. It is composed of 1204 amino acids and has a molecular weight of approximately 136 kDa. The protein consists of two major domains – the N-terminal cytoplasmic domain and the C-terminal catalytic domain. The N-terminal domain is responsible for the regulation of enzyme activity, while the C-terminal domain contains the catalytic site where the conversion of ATP to cAMP takes place.

The N-His tag is a short sequence of six histidine residues that has been added to the C-terminus of the protein. This tag allows for easy purification and detection of the recombinant protein. The addition of the N-His tag does not alter the structure or function of the protein, making it an ideal tool for research and drug development.

Activity of this protein

Recombinant Human ADCY9, N-His is an enzyme that catalyzes the conversion of ATP to cyclic adenosine monophosphate (cAMP). cAMP is an important signaling molecule that regulates various cellular processes such as metabolism, gene expression, and cell growth. The activity of ADCY9 is regulated by various factors, including hormones, neurotransmitters, and calcium ions.

Studies have shown that the activity of ADCY9 is crucial for the development and function of the nervous system. It has been found to play a role in learning and memory, as well as in the regulation of mood and behavior. Dysregulation of ADCY9 activity has also been linked to various diseases, including depression, schizophrenia, and Parkinson’s disease.

Application of Recombinant Human ADCY9, N-His

Recombinant Human ADCY9, N-His has many potential applications in both research and drug development. As a drug target, it has been identified as a potential therapeutic target for various diseases, particularly those related to the nervous system. By modulating the activity of ADCY9, it may be possible to treat conditions such as depression, anxiety disorders, and neurodegenerative diseases.

In research, Recombinant Human ADCY9, N-His can be used to study the structure and function of the native protein. It can also be used to screen for potential drug candidates that can modulate its activity. The N-His tag also allows for easy purification and detection of the protein, making it a valuable tool for studying its role in cellular signaling pathways.

In conclusion, Recombinant Human ADCY9, N-His is a protein with a similar structure and activity to the native human ADCY9 protein. Its activity is crucial for the regulation of cellular signaling pathways and has been identified as a potential drug target for various diseases. With its potential applications in research and drug development, this protein holds great promise for the treatment of diseases related to the nervous system.