Recombinant Human AKR1B10, N-His

Description of Recombinant Human AKR1B10, N-His

Introduction

Recombinant Human AKR1B10, N-His is a protein that plays a crucial role in various biological processes, including metabolism, detoxification, and cell proliferation. This protein belongs to the aldo-keto reductase (AKR) superfamily and is highly expressed in several human tissues, making it a potential drug target for various diseases. In this article, we will discuss the structure, activity, and application of Recombinant Human AKR1B10, N-His.

Structure of Recombinant Human AKR1B10, N-His

The AKR1B10 gene is located on chromosome 7 and encodes a protein of 316 amino acids. The recombinant form of this protein is produced using genetic engineering techniques and contains an additional N-terminal histidine tag (N-His). This tag allows for easy purification and detection of the protein.

The crystal structure of Recombinant Human AKR1B10, N-His has been determined, revealing a compact three-dimensional structure with a central beta-sheet surrounded by alpha-helices. The active site of the protein is located in a deep pocket, which can accommodate small molecules, such as substrates and inhibitors.

Activity of this protein

Recombinant Human AKR1B10, N-His is an NADPH-dependent enzyme that catalyzes the reduction of various substrates, including aldehydes, ketones, and steroids. This protein is highly specific for substrates with a carbonyl group, and its activity is regulated by the availability of NADPH.

One of the main functions of Recombinant Human AKR1B10, N-His is the detoxification of reactive carbonyl species, which are produced as by-products of cellular metabolism. This protein plays a crucial role in protecting cells against oxidative stress and maintaining redox balance.

Application of Recombinant Human AKR1B10, N-His

Due to its diverse substrate specificity and high expression in various human tissues, Recombinant Human AKR1B10, N-His has been identified as a potential drug target for several diseases. Here are some of its applications:

1. Cancer
Recombinant Human AKR1B10, N-His is overexpressed in various types of cancer, including liver, lung, and breast cancer. Its role in cell proliferation and detoxification makes it an attractive target for cancer therapy. Inhibitors of this protein have been developed and are currently being evaluated in pre-clinical and clinical trials.

2. Diabetes
AKR1B10 has been implicated in the development of diabetic complications, such as retinopathy and neuropathy. Inhibition of this protein has shown promising results in animal models, making it a potential target for the treatment of diabetes.

3. Inflammation
Inflammation is a common feature of many diseases, and Recombinant Human AKR1B10, N-His has been shown to play a role in regulating the inflammatory response. Inhibition of this protein has been proposed as a potential treatment for inflammatory diseases, such as rheumatoid arthritis and inflammatory bowel disease.

4. Drug metabolism
Recombinant Human AKR1B10, N-His is involved in the metabolism of several drugs, including anticancer agents and steroids. Inhibition of this protein can alter the efficacy and toxicity of these drugs, making it a potential target for drug development and personalized medicine.

Conclusion

Recombinant Human AKR1B10, N-His is a versatile protein with important roles in various biological processes. Its structure, activity, and potential as a drug target make it an attractive molecule for further research and development. In the future, we can expect to see more applications of this protein in the treatment of various diseases.