Recombinant Human DUSP11, N-His

Description of Recombinant Human DUSP11, N-His

Introduction

Recombinant Human DUSP11, N-His is a protein that has gained significant attention as a potential drug target due to its role in regulating cellular signaling pathways. This protein belongs to the dual-specificity phosphatase (DUSP) family and plays a crucial role in dephosphorylating both tyrosine and serine/threonine residues on target proteins. In this article, we will discuss the structure, activity, and potential applications of Recombinant Human DUSP11, N-His.

Structure of Recombinant Human DUSP11, N-His

The human DUSP11 gene is located on chromosome 1 and encodes for a protein of 377 amino acids. The recombinant form of this protein, known as Recombinant Human DUSP11, N-His, is produced through genetic engineering techniques and contains an additional N-terminal His-tag for purification and detection purposes.

The crystal structure of Recombinant Human DUSP11, N-His has been determined and revealed that it adopts a typical DUSP fold, consisting of a central α/β core surrounded by two α-helices and two β-strands. The active site of this protein contains a conserved cysteine residue, which is essential for its phosphatase activity.

Activity of this protein

Recombinant Human DUSP11, N-His is a dual-specificity phosphatase that can dephosphorylate both tyrosine and serine/threonine residues on target proteins. This activity is crucial for regulating various signaling pathways, including the MAPK and JNK pathways, which are involved in cell proliferation, differentiation, and survival.

Studies have shown that Recombinant Human DUSP11, N-His can specifically dephosphorylate the MAPK kinases MEK1 and MEK2, thereby inhibiting the downstream activation of the ERK1/2 pathway. This inhibition of ERK1/2 signaling has been linked to the anti-proliferative effects of Recombinant Human DUSP11, N-His in cancer cells.

Applications of Recombinant Human DUSP11, N-His

Given its role in regulating important signaling pathways, Recombinant Human DUSP11, N-His has potential applications in various fields, including cancer research and drug development. As mentioned earlier, its ability to inhibit ERK1/2 signaling makes it a promising candidate for cancer treatment.

In addition, Recombinant Human DUSP11, N-His has also been shown to play a role in immune response regulation. Studies have demonstrated that this protein can modulate the activity of T cells, which are essential for the body’s defense against pathogens. Therefore, it has potential applications in autoimmune diseases and transplant rejection.

Furthermore, Recombinant Human DUSP11, N-His has also been studied for its role in neurodegenerative diseases, such as Alzheimer’s and Parkinson’s disease. It has been shown to regulate the activity of proteins involved in the formation of protein aggregates, which are a hallmark of these diseases.

Conclusion

In summary, Recombinant Human DUSP11, N-His is a promising protein with potential applications in various fields, including cancer research, immunology, and neurodegenerative diseases. Its unique dual-specificity phosphatase activity and role in regulating important signaling pathways make it an attractive drug target for future research and development.