Recombinant Human ROCK2, N-His

Description of Recombinant Human ROCK2, N-His

Introduction

Recombinant Human ROCK2, N-His is a protein that plays a crucial role in various cellular processes, making it an important drug target for the treatment of many diseases. In this article, we will explore the structure, activity, and potential applications of this protein.

Structure of Recombinant Human ROCK2, N-His

ROCK2, N-His is a member of the Rho-associated coiled-coil containing protein kinases (ROCK) family. It is a 160 kDa protein that consists of 1382 amino acids. The protein has a unique structure, with a kinase domain at the N-terminus and a coiled-coil domain at the C-terminus. The kinase domain is responsible for the catalytic activity of the protein, while the coiled-coil domain is involved in protein-protein interactions.

ROCK2, N-His also contains a His-tag at the N-terminus, which allows for easy purification and detection of the protein. This tag does not interfere with the protein’s activity, making it a useful tool for research and drug development.

Activity of this protein

ROCK2, N-His is a serine/threonine kinase, meaning it catalyzes the transfer of a phosphate group from ATP to serine or threonine residues on target proteins. This activity is crucial for the regulation of various cellular processes, including cell proliferation, migration, and contraction.

One of the main functions of ROCK2, N-His is its role in the RhoA/ROCK signaling pathway. This pathway is involved in the regulation of actin cytoskeleton, which is essential for cell movement and shape. ROCK2, N-His phosphorylates and activates downstream targets, such as myosin light chain (MLC), leading to actin cytoskeleton reorganization and cell contraction.

Besides its role in the RhoA/ROCK pathway, ROCK2, N-His also plays a role in other signaling pathways, such as the Wnt/β-catenin pathway, which is involved in cell proliferation and differentiation. Dysregulation of these pathways has been linked to various diseases, making ROCK2, N-His a potential drug target for their treatment.

Applications of Recombinant Human ROCK2, N-His

Due to its crucial role in cellular processes, ROCK2, N-His has been identified as a potential drug target for the treatment of various diseases. One of the main applications of this protein is in the treatment of cancer. ROCK2, N-His has been found to be overexpressed in many types of cancer, and its inhibition has been shown to suppress cancer cell growth and invasion. Therefore, inhibitors of ROCK2, N-His are being developed as potential anticancer drugs.

ROCK2, N-His has also been implicated in other diseases, such as cardiovascular diseases, neurological disorders, and autoimmune diseases. Inhibition of ROCK2, N-His has shown promising results in preclinical studies for these diseases, making it a potential target for drug development.

Besides its potential as a drug target, recombinant Human ROCK2, N-His is also useful in research. Its His-tag allows for easy purification and detection, making it a valuable tool for studying the RhoA/ROCK signaling pathway and other cellular processes involving ROCK2.

Conclusion

In conclusion, Recombinant Human ROCK2, N-His is a crucial protein involved in various cellular processes. Its unique structure and activity make it a potential drug target for the treatment of cancer and other diseases. Its applications in research also make it a valuable tool for studying cellular signaling pathways. Further research and drug development targeting ROCK2, N-His could lead to new treatments for a wide range of diseases.