Recombinant Human STAT1, N-His

Description of Recombinant Human STAT1, N-His

Introduction

Recombinant Human STAT1, N-His is a protein that plays a crucial role in the regulation of immune responses and cell growth. It is a drug target for various diseases and is widely used in research and therapeutic applications. In this article, we will explore the structure, activity, and applications of Recombinant Human STAT1, N-His.

Structure of Recombinant Human STAT1, N-His

Recombinant Human STAT1, N-His is a member of the signal transducer and activator of transcription (STAT) family of proteins. It is a 91 kDa protein composed of 750 amino acids. The N-His tag is a short sequence of six histidine residues added to the N-terminus of the protein. This tag allows for easy purification and detection of the protein.

The protein consists of seven functional domains, including the N-terminal domain, coiled-coil domain, DNA-binding domain, linker domain, SH2 domain, transactivation domain, and C-terminal domain. These domains play a crucial role in the protein’s activity and function.

Activity of Recombinant Human STAT1, N-His

Recombinant Human STAT1, N-His is a transcription factor that is activated in response to cytokines and growth factors. When stimulated, the protein forms a homodimer and translocates to the nucleus, where it binds to specific DNA sequences, known as IFN-γ activated sites (GAS). This binding leads to the activation of target genes involved in immune responses, cell proliferation, and differentiation.

Moreover, Recombinant Human STAT1, N-His is also involved in the regulation of the JAK-STAT signaling pathway. It is phosphorylated by Janus kinases (JAKs) upon cytokine stimulation, and this phosphorylation is essential for its transcriptional activity.

Applications of this protein

Drug Target for Diseases

Recombinant Human STAT1, N-His is a crucial drug target for various diseases, including cancer, viral infections, and autoimmune disorders. Dysregulation of STAT1 has been linked to the development and progression of these diseases. Targeting this protein with specific inhibitors can potentially lead to the development of new therapies.

Research Tool

Recombinant Human STAT1, N-His is widely used as a research tool to study the JAK-STAT signaling pathway and its role in immune responses and cell growth. The protein is available in a purified and active form, making it suitable for a wide range of experiments, including protein-protein interaction studies, DNA binding assays, and gene expression analysis.

Therapeutic Applications

Recombinant Human STAT1, N-His has therapeutic potential in the treatment of various diseases. For example, it has been used in the treatment of chronic granulomatous disease (CGD), a rare genetic disorder characterized by recurrent bacterial and fungal infections. Recombinant Human STAT1, N-His can enhance the immune response in CGD patients and improve their overall health.

Moreover, the protein has also been investigated for its potential in cancer therapy. Studies have shown that targeting STAT1 can inhibit tumor growth and enhance the efficacy of chemotherapy drugs.

Conclusion

Recombinant Human STAT1, N-His is a crucial protein involved in immune responses and cell growth. Its structure, activity, and applications make it a valuable drug target, research tool, and potential therapeutic agent. Further research on this protein can lead to the development of new treatments for various diseases.