Recombinant Human SUZ12 Protein, N-His

Description of Recombinant Human SUZ12 Protein, N-His

Introduction

Recombinant Human SUZ12 Protein is a key component of the Polycomb Repressive Complex 2 (PRC2), which plays a crucial role in epigenetic gene regulation. This protein is highly conserved across species and has been extensively studied due to its involvement in various cellular processes. In this article, we will discuss the structure, activity, and applications of Recombinant Human SUZ12 Protein.

Structure of Recombinant Human SUZ12 Protein

Recombinant Human SUZ12 Protein is a 739-amino acid protein with a molecular weight of approximately 83 kDa. It contains a conserved zinc finger domain and a C-terminal VEFS box, which are essential for its function in the PRC2 complex. The zinc finger domain is responsible for binding to the histone H3 lysine 27 (H3K27) residue, while the VEFS box interacts with other PRC2 subunits.

In addition, SUZ12 has a highly conserved SANT (SWI3, ADA2, N-CoR, and TFIIIB) domain, which is involved in protein-protein interactions and DNA binding. This domain is crucial for the recruitment of SUZ12 to specific target genes and for its enzymatic activity.

Activity of Recombinant Human SUZ12 Protein

Recombinant Human SUZ12 Protein is a core component of the PRC2 complex, which is responsible for the trimethylation of H3K27. This post-translational modification results in the formation of a repressive chromatin state, leading to the silencing of target genes. SUZ12 plays a critical role in this process by recognizing and binding to specific DNA sequences via its SANT domain and recruiting the PRC2 complex to these sites.

Moreover, SUZ12 also interacts with other PRC2 subunits, such as EZH2 and EED, to stabilize the complex and enhance its enzymatic activity. This interaction is essential for the proper functioning of PRC2 and the maintenance of gene silencing.

Applications of Recombinant Human SUZ12 Protein

Due to its crucial role in epigenetic gene regulation, Recombinant Human SUZ12 Protein has various applications in both research and medicine. Here are some of the key applications of this protein:

1. Understanding epigenetic mechanisms

Recombinant Human SUZ12 Protein has been extensively used in research to study the mechanisms of epigenetic gene regulation. By studying its structure and activity, scientists can gain insights into how this protein and the PRC2 complex function in the cell. This knowledge can help in understanding the role of epigenetics in various diseases and in developing potential treatments.

2. Biomarker for cancer diagnosis and prognosis

The PRC2 complex has been implicated in various types of cancer, and SUZ12 is often found to be overexpressed in these tumors. As a result, Recombinant Human SUZ12 Protein has been used as a biomarker for cancer diagnosis and prognosis. Its expression levels can help in identifying patients who are at a higher risk of developing cancer and in predicting the outcome of the disease.

3. Target for drug development

Given the critical role of SUZ12 in epigenetic gene regulation and its involvement in cancer, it has emerged as a potential target for drug development. Researchers are currently exploring various compounds that can inhibit the activity of SUZ12 or disrupt its interaction with other PRC2 subunits. These drugs could potentially be used to treat cancer and other diseases associated with abnormal epigenetic regulation.

4. Production of recombinant antibodies

Recombinant Human SUZ12 Protein has also been used as an antigen to produce specific antibodies for research purposes. These antibodies can be used to study the expression and localization of SUZ12 in different cell types and tissues, providing valuable insights into its function.

Conclusion

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