Recombinant Human ZNF671 Protein, N-His

Description of Recombinant Human ZNF671 Protein, N-His

Introduction

Recombinant Human ZNF671 Protein, also known as Zinc Finger Protein 671, is a protein that is encoded by the ZNF671 gene in humans. It belongs to the C2H2-type zinc finger protein family and plays a crucial role in various cellular processes. In this article, we will discuss the structure, activity, and applications of this recombinant protein.

Structure of Recombinant Human ZNF671 Protein

The ZNF671 gene is located on chromosome 19 and consists of 5 exons. The protein is composed of 365 amino acids and has a molecular weight of approximately 40 kDa. It contains 7 C2H2-type zinc finger domains, which are responsible for its DNA-binding activity. These zinc finger domains are arranged in a tandem pattern and are connected by short linker sequences. The structure of ZNF671 protein is highly conserved among different species, indicating its importance in biological processes.

Activity of Recombinant Human ZNF671 Protein

Recombinant Human ZNF671 Protein is a transcription factor that regulates gene expression by binding to specific DNA sequences. It has been shown to bind to GC-rich regions of DNA and can either activate or repress gene expression depending on the target gene. This protein has been found to play a role in various cellular processes such as cell proliferation, differentiation, and apoptosis. It also interacts with other proteins to form complexes that regulate gene expression.

Applications of Recombinant Human ZNF671 Protein

Recombinant Human ZNF671 Protein has a wide range of applications in both research and medical fields. Some of the major applications are listed below:

1. Recombinant Protein Production

Recombinant Human ZNF671 Protein can be produced in large quantities using recombinant DNA technology. This protein can be expressed in different host systems such as bacteria, yeast, and mammalian cells. It is used as a tool in various biochemical and biophysical studies due to its ability to bind to specific DNA sequences.

2. Antigen for Antibody Production

Recombinant Human ZNF671 Protein can be used as an antigen to produce specific antibodies. These antibodies can be used for various applications such as immunohistochemistry, western blotting, and immunoprecipitation. They can also be used in diagnostic tests to detect the presence of ZNF671 protein in biological samples.

3. Functional Studies

Recombinant Human ZNF671 Protein can be used in functional studies to understand its role in different biological processes. Its DNA-binding activity can be studied using techniques such as electrophoretic mobility shift assay (EMSA) and chromatin immunoprecipitation (ChIP). These studies can provide insights into the molecular mechanisms of gene regulation by ZNF671 protein.

4. Therapeutic Applications

Aberrant expression of ZNF671 protein has been linked to various diseases such as cancer and neurological disorders. Recombinant Human ZNF671 Protein can be used in drug discovery and development to target this protein and potentially treat these diseases. It can also be used as a biomarker for disease diagnosis and prognosis.

5. Gene Therapy

Recombinant Human ZNF671 Protein can be used in gene therapy to regulate gene expression in specific cells or tissues. This can be achieved by using ZNF671 protein as a transcriptional activator or repressor, depending on the target gene. This approach has potential applications in treating genetic disorders and other diseases caused by abnormal gene expression.

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