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E. Coli protein expression service

A tailored workflow for your protein project 

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Purified protein, delivered at the quantity you need

36 expression test conditions

Scalable and reproducible production up to 120L

Determine the best protein expression conditions through a 36-test screening approach

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Start with a preliminary study designed to identify the best expression strategy for your protein. Using a 36-test screening approach, we evaluate the most suitable expression conditions for your project to optimize yield and cost. At the end of the process, you receive purified protein samples in your required quantity, ready to support your next development steps.

 

Start your protein project

A custom E. coli protein expression service tailored to your project for better outcomes

Your project begins with a preliminary study designed to define the best strategy for your custom protein. A dedicated expert supports you throughout the workflow and adjusts each step to your specific requirements and constraints.

Expression vector construction

  • Gene design, including codon optimization
  • Gene subcloning into a high-yield expression vector
  • Multiple vector systems available

Preliminary study: screening of 36 conditions and optimization to assess production parameters and determine the best protein expression conditions

Protein expression scale-up and purification

  • Minimum 200 mL culture volume for protein expression under the previously optimized conditions
  • 3 purification methods: affinity chromatography, size exclusion chromatography, ion exchange chromatography

Optional or alternative steps

Quality control analytics

  • SDS-PAGE and Western Blot analysis
  • Other optional methods: stability test, buffer screening, structure analysis, ….

Define the best strategy with our experts

Data report

Get free access to representative data 
from a E.coli Expression & purification test project

You receive a comprehensive report with detailed data, advanced test results, and key insights throughout the process.

Download the Data Report

Expert solutions for complex proteins to maximize your chances of success

Our custom strategies and specialized techniques help you obtain functional proteins, even for complex projects, to maximize your chances of success. For proteins from inclusion bodies, our experts select the most suitable of two refolding methods (urea or sarkosyl) based on the specific properties of your protein.

With our protein characterization services, you gain clearer insight into protein properties to define the strategy best suited to your project.

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Your protein of interest could be ready to order

 

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 A reproducible scale-up for your protein project

Your protein production can be scaled up from microgram to gram quantities through a wide range of volumes, up to 120 L, with a single partner. You benefit from a reproducible path to the quantity needed for your project.

Our approach can support custom cell line development to enable industrial-scale production, which requires a switch to a mammalian expression system.

 

Discuss with us to discover how you can scale-up your protein

 

Feedback on our E. coli protein expression expertise

“ProteoGenix assisted me with the generation of a peptide and a protein. They were very helpful throughout the entire process, and provided invaluable information and recommendations based on their expertise. Based on this experience, I look forward to future collaborations with ProteoGenix ” – Karina ARREGUIN, Augusta University

“ ProteoGenix is for us a reliable partner for the production of recombinant proteins in E.coli and always provided an excellent service, with regular updates, quick response to our queries and delivery of product in high quality. We appreciate the collaborative attitude and professionalism of ProteoGenix.” – M. Napieralski Rudolf, Therawis Diagnostics GmbH

“ProteoGenix is a reliable partner for the production of recombinant protein in E. Coli. I appreciated the collaborative and prompt problem solving attitude of their account managers, which definitely added value to our collaboration.” – Gabriel Krouk, Research Director CNRS

“We were very pleased not only by the professional and prompt customer service, but also by the rapid turnaround and handling of the two proteins we asked to be produced in E. Coli. We were guided through all stages of the process and afterwards we received our proteins ready to start our screening program.” – Prof. Steven Ballet, Vrije Universiteit Brussel

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