Recombinant Human ATG14 Protein, N-His

Description of Recombinant Human ATG14 Protein, N-His

Introduction to Recombinant Human ATG14 Protein

Recombinant Human ATG14 Protein, also known as Autophagy Related Protein 14, is a key component of the autophagy pathway. This protein is crucial for the formation of the autophagosome, a double-membrane vesicle that plays a critical role in the degradation of cellular components. Recombinant Human ATG14 Protein is a highly conserved protein found in all eukaryotic organisms, and its function is essential for maintaining cellular homeostasis.

Structure of Recombinant Human ATG14 Protein

Recombinant Human ATG14 Protein is a 53 kDa protein composed of 467 amino acids. It contains a coiled-coil domain at the N-terminus, which is essential for its interaction with other proteins involved in the autophagy pathway. The C-terminus of the protein contains a WD40 repeat domain, which is responsible for binding to membrane lipids and regulating the activity of the protein.

The crystal structure of Recombinant Human ATG14 Protein has been determined, revealing important insights into its function. The coiled-coil domain forms a dimer, which is essential for the proper function of the protein. The WD40 repeat domain contains a hydrophobic pocket that allows for the binding of lipids, specifically phosphatidylinositol 3-phosphate (PI3P), a key regulator of autophagy.

Activity of Recombinant Human ATG14 Protein

Recombinant Human ATG14 Protein is a critical component of the autophagy pathway, which is responsible for the degradation of cellular components. The activity of this protein is tightly regulated, and its function is essential for maintaining cellular homeostasis.

Recombinant Human ATG14 Protein is involved in the initial stages of autophagosome formation. It acts as a scaffold protein, bringing together other key autophagy proteins, such as ULK1, VPS34, and Beclin-1, to form the pre-autophagosomal structure (PAS). This structure serves as the site of autophagosome formation and is essential for the proper functioning of the autophagy pathway.

Additionally, Recombinant Human ATG14 Protein is involved in the regulation of PI3P, a key lipid that is essential for autophagosome formation. It binds to PI3P and helps to recruit other proteins to the PAS, further promoting the formation of the autophagosome.

Application of Recombinant Human ATG14 Protein

The role of autophagy in cellular homeostasis has been extensively studied, and dysregulation of this pathway has been linked to various diseases, including cancer, neurodegenerative disorders, and metabolic diseases. As such, Recombinant Human ATG14 Protein has significant potential as a therapeutic target.

Furthermore, Recombinant Human ATG14 Protein has been used in research studies to better understand the role of autophagy in various diseases. It has been shown that mutations in the gene encoding ATG14 can lead to defects in autophagy and contribute to the development of diseases such as Crohn’s disease and colorectal cancer.

Recombinant Human ATG14 Protein has also been used in the development of diagnostic tools for autophagy-related diseases. By studying the activity of this protein, researchers can gain insights into the functioning of the autophagy pathway and its role in disease development.

Conclusion

In summary, Recombinant Human ATG14 Protein is a crucial component of the autophagy pathway, involved in the formation of the autophagosome and regulation of PI3P. Its structure, activity, and potential applications make it a valuable tool for understanding the role of autophagy in various diseases and as a potential therapeutic target. Continued research on this