Recombinant Human CDC25C Protein, N-His

Description of Recombinant Human CDC25C Protein, N-His

Recombinant Human CDC25C Protein: Structure, Activity, and Application

Introduction

The CDC25C protein is a key regulator of the cell cycle, playing a crucial role in the progression from G2 to M phase. It is a member of the CDC25 family of dual-specificity phosphatases, which are responsible for dephosphorylating and activating cyclin-dependent kinases (CDKs) at specific points in the cell cycle. The human CDC25C gene has been cloned and expressed in various systems, resulting in the production of recombinant human CDC25C protein. In this article, we will explore the structure, activity, and application of this important protein.

Structure of Recombinant Human CDC25C Protein

The recombinant human CDC25C protein is a 55 kDa polypeptide consisting of 473 amino acids. It contains a conserved catalytic domain, as well as a C-terminal regulatory domain. The catalytic domain is responsible for the dephosphorylation activity of the protein, while the regulatory domain plays a role in substrate specificity and protein-protein interactions.

The crystal structure of recombinant human CDC25C protein has been determined, revealing a compact and highly conserved protein fold. The catalytic domain adopts a dual-specificity phosphatase fold, with a central β-sheet surrounded by α-helices. The regulatory domain is located at the C-terminus and contains a unique loop structure that is important for substrate recognition.

Activity of Recombinant Human CDC25C Protein

The main function of recombinant human CDC25C protein is to activate CDKs by dephosphorylating specific residues. This allows for the transition from G2 to M phase in the cell cycle. The activity of CDC25C is tightly regulated by phosphorylation and interaction with other proteins. In its active form, CDC25C is able to dephosphorylate and activate CDK1, which is essential for the initiation of mitosis.

In addition to its role in the cell cycle, recombinant human CDC25C protein has been shown to have other important functions. It has been implicated in DNA damage repair, as well as in the regulation of cell survival and apoptosis. Its activity has also been linked to various diseases, including cancer, making it an important target for drug development.

Application of Recombinant Human CDC25C Protein

The production of recombinant human CDC25C protein has allowed for a better understanding of its structure and function. It has also been used in various in vitro and in vivo studies to investigate its role in the cell cycle and other cellular processes. In addition, recombinant CDC25C protein has been used as an antigen to develop specific antibodies for research and diagnostic purposes.

Furthermore, the potential of CDC25C as a therapeutic target has led to the development of small molecule inhibitors that can specifically block its activity. These inhibitors have shown promising results in pre-clinical studies, making CDC25C an attractive target for cancer treatment.

Conclusion

The recombinant human CDC25C protein is a crucial player in the cell cycle, with important functions in cell division, DNA damage repair, and cell survival. Its structure and activity have been extensively studied, and its potential as a therapeutic target has led to the development of specific inhibitors. The production of recombinant CDC25C protein has greatly contributed to our understanding of its role in cellular processes and has opened up new avenues for research and drug development.