Recombinant Human NCBP1 Protein, N-His

Description of Recombinant Human NCBP1 Protein, N-His

Introduction to Recombinant Human NCBP1 Protein

Recombinant Human NCBP1 Protein, also known as nuclear cap-binding protein subunit 1, is a protein that plays a crucial role in the regulation of gene expression. This protein is encoded by the NCBP1 gene and is a component of the nuclear cap-binding complex (CBC). The CBC is responsible for binding to the 5′ cap structure of messenger RNA (mRNA), which is essential for efficient translation of the mRNA into protein. Recombinant Human NCBP1 Protein is produced through genetic engineering techniques, making it a valuable tool in various scientific and medical applications.

Structure of Recombinant Human NCBP1 Protein

Recombinant Human NCBP1 Protein is a 62-kDa protein that consists of 576 amino acids. It contains a highly conserved N-terminal domain, a central RNA-binding domain, and a C-terminal domain. The N-terminal domain is responsible for binding to the cap structure of mRNA, while the RNA-binding domain interacts with the poly(A) tail of mRNA. The C-terminal domain is involved in protein-protein interactions and is essential for the stability of the CBC complex. Recombinant Human NCBP1 Protein also contains several phosphorylation sites, which play a role in regulating its activity.

Activity of Recombinant Human NCBP1 Protein

The main function of Recombinant Human NCBP1 Protein is to bind to the cap structure of mRNA and facilitate its translation into protein. This protein is essential for the efficient translation of all mRNAs, as it ensures that the mRNA is properly positioned on the ribosome. In addition to its role in translation, Recombinant Human NCBP1 Protein also plays a role in mRNA stability and nuclear export. It has been shown to interact with other proteins involved in these processes, such as the poly(A) binding protein (PABP) and the nuclear export factor 1 (NXF1).

Applications of Recombinant Human NCBP1 Protein

Recombinant Human NCBP1 Protein has a wide range of applications in both research and medical fields. Its ability to bind to the cap structure of mRNA makes it a valuable tool for studying gene expression and translation. It can be used to investigate the role of the CBC complex in various cellular processes, such as mRNA splicing and nuclear export. Recombinant Human NCBP1 Protein can also be used to study the regulation of gene expression in different disease states, such as cancer.

In addition to its research applications, Recombinant Human NCBP1 Protein has potential therapeutic uses. It has been shown to have anti-tumor effects in certain types of cancer, and research is ongoing to explore its potential as a cancer treatment. This protein has also been investigated as a possible target for antiviral therapies, as it plays a role in the replication of certain viruses.

Conclusion

Recombinant Human NCBP1 Protein is a vital component of the CBC complex, which plays a crucial role in gene expression and translation. Its structure, activity, and applications make it a valuable tool for studying various cellular processes and diseases. As research continues to uncover the functions of this protein, it has the potential to be used in a variety of therapeutic interventions.