Recombinant Human RAB9A Protein, N-His

Description of Recombinant Human RAB9A Protein, N-His

The Structure and Function of Recombinant Human RAB9A Protein

Recombinant Human RAB9A Protein, also known as Ras-related protein Rab-9A, is a small GTPase protein that plays a crucial role in intracellular trafficking and vesicle transport. It is encoded by the RAB9A gene and is a member of the RAB family of proteins, which are involved in regulating various cellular processes such as endocytosis, exocytosis, and vesicle fusion.

Structure of Recombinant Human RAB9A Protein

The RAB9A gene is located on chromosome 19 in humans and is approximately 2.5 kb in length. The protein is composed of 205 amino acids with a molecular weight of 23 kDa. It has a conserved GTP-binding domain at the N-terminus and a C-terminal hypervariable region that is responsible for protein-protein interactions.

The crystal structure of Recombinant Human RAB9A Protein has been determined, revealing that it adopts a globular shape with six α-helices and six β-strands. It also contains a bound nucleotide, either GTP or GDP, which is essential for its activity.

Activity of Recombinant Human RAB9A Protein

Recombinant Human RAB9A Protein is a key regulator of the late endosomal/lysosomal pathway, which is responsible for the degradation of cellular components. It is involved in the transport of cargo from early endosomes to late endosomes, and then to lysosomes for degradation.

Upon activation by GTP binding, Recombinant Human RAB9A Protein interacts with effector proteins, such as the tethering factor RILP, to promote the fusion of late endosomes with lysosomes. This process is essential for maintaining the proper function of the endosomal/lysosomal system and for the degradation of various cellular components, including proteins, lipids, and carbohydrates.

Application of Recombinant Human RAB9A Protein

Due to its crucial role in intracellular trafficking, Recombinant Human RAB9A Protein has been widely used in various research studies to better understand the mechanisms of endosomal/lysosomal function and vesicle transport. It has also been implicated in several diseases, including neurodegenerative disorders and infectious diseases, making it a potential target for therapeutic interventions.

Recombinant Human RAB9A Protein is also used in drug discovery and development, as it is involved in the trafficking of receptors and transporters that are targeted by many drugs. By studying its activity and interactions with other proteins, researchers can gain insights into the mechanisms of drug action and identify potential new drug targets.

Moreover, Recombinant Human RAB9A Protein has been used in the development of diagnostic tools for certain diseases. For example, it has been shown to be a potential biomarker for Alzheimer’s disease, as its levels have been found to be altered in the brains of patients with this condition. This opens up the possibility of using Recombinant Human RAB9A Protein as a diagnostic tool for early detection and monitoring of disease progression.

Conclusion

Recombinant Human RAB9A Protein is a crucial protein involved in the regulation of intracellular trafficking and vesicle transport. Its structure and function have been extensively studied, and its role in various cellular processes and diseases has been well-established. With its potential applications in research, drug development, and diagnostics, Recombinant Human RAB9A Protein continues to be an important protein in the scientific community.