Recombinant Monkeypox virus/MPXV A35R Protein

Reference: PX-P6034-100
Product nameRecombinant Monkeypox virus/MPXV A35R Protein
Origin speciesMonkeypox virus (strain Zaire-96-I-16) (MPX)
Expression systemEukaryotic expression
Molecular weight16.65 kDa
Protein delivered with Tag?C-terminal His Tag
Purity estimated>85% by SDS-PAGE
BufferPBS, pH7.5
Delivery conditionDry Ice
Delivery lead time in business days3-5 days if in stock; 3-5 weeks if production needed
Storage condition4°C for short term (1 week), -20°C or -80°C for long term (avoid freezing/thawing cycles; addition of 20-40% glycerol improves cryoprotection)
BrandProteoGenix
Host speciesMammalian cells
ApplicationsELISA,WB
Fragment TypeVal57-Thr181
Aliases /SynonymsMonkeypox A35R, clade 2
ReferencePX-P6034
NoteFor research use only.

SDS-PAGE For Recombinant Monkeypox virus/MPXV A35R Protein

Recombinant Monkeypox virus/MPXV A35R Protein, on SDS-PAGE under reducing condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Anti-MPXV A35R Antibody (SAA0287) binds with A35R protein in ELISA assay

Immobilized Recombinant Monkeypox virus/MPXV A35R Protein (cat. No. PX-P6034) at 0.5µg/mL (100µL/well) can bind Monkeypox virus/MPXV A35R Monoclonal Antibody SAA0287 (cat. No. PTX18825) in indirect ELISA with Goat Anti-Human IgG secondary antibody coupled with HRP measured by OD450 giving an EC50 at 31.66M.

Publication

Daniel Stern, Rebecca Surtees, Fridolin Treindl et al. Differentiation between mpox infection and MVA immunization by a novel machine learning-supported serological multiplex assay, 09 August 2024, PREPRINT (Version 1) available at Research Square [https://doi.org/10.21203/rs.3.rs-4822502/v1]

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