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Immobilized Human TNFa Recombinant Protein (cat. No.PX-P3058) at 0.5µg/mL (100µL/well) can bind Adalimumab Biosimilar - Anti-TNF alpha mAb (cat. No.PX-TA1001) in indirect ELISA with Goat Anti-Human IgG secondary antibody coupled with HRP measured by OD450 giving an EC50 at 142.1M.
Adalimumab Biosimilar - Anti-TNF alpha mAb, on SDS-PAGE under reducing and non-reducing condition. The gel was stained overnight with Coomassie Blue. The purity of the antibody is greater than 95%.
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Ofer Gover — December 14, 2021
★★★★★
We used this ab to reduce as a negative control for reducing caco-2 (IEC) cells exitation by exugenues TNFa. We measured IL8 secreation as a result of TNFa stimulation. 1ug/mL ofAdalimumab Biosimilar – Anti-TNF alpha mAb was enough to completly abolish IL8 secreation by TNFa stimulation in caco-2 cells.