HIRA protein, Human HIRA(155-254) Recombinant Protein

Description of HIRA protein / Human HIRA(155-254) Recombinant Protein

Introduction

The HIRA protein, also known as the Histone Cell Cycle Regulation Defective Homolog A, is an essential component of the human chromatin remodeling complex. This protein plays a crucial role in regulating the structure and function of chromatin, which is the complex of DNA and proteins that make up the genetic material in our cells. In this article, we will discuss the structure, activity, and potential applications of the Human HIRA(155-254) Recombinant Protein, which is a truncated form of the full-length HIRA protein.

Structure of HIRA Protein

The HIRA protein is a large protein with a molecular weight of approximately 120 kDa. It is composed of 1042 amino acids and contains several functional domains, including a WD40 repeat domain, a PHD finger domain, and a HIRAN domain. The WD40 repeat domain is responsible for protein-protein interactions, while the PHD finger domain is involved in binding to histone proteins. The HIRAN domain is thought to be important for the recruitment of HIRA to specific regions of chromatin.

The Human HIRA(155-254) Recombinant Protein is a truncated form of the full-length HIRA protein, consisting of amino acids 155-254. This truncated form retains the PHD finger domain, which is essential for its activity, but lacks the WD40 repeat and HIRAN domains. This truncated form is often used in scientific research as it is easier to produce and purify compared to the full-length protein.

Activity of HIRA Protein

The HIRA protein is involved in several important cellular processes, including chromatin remodeling, DNA repair, and gene expression regulation. It is known to interact with various proteins and complexes, including the histone chaperone ASF1, the nucleosome remodeling and deacetylase (NuRD) complex, and the polycomb repressive complex 2 (PRC2). Through these interactions, HIRA is able to regulate the structure and function of chromatin.

One of the key functions of HIRA is its role in the deposition of histone variant H3.3 onto chromatin. This histone variant is important for maintaining the integrity of the genome and is associated with active gene expression. HIRA helps to deposit H3.3 onto newly replicated DNA, ensuring that the newly synthesized DNA is properly packaged and accessible for gene expression.

Potential Applications of Human HIRA(155-254) Recombinant Protein

The Human HIRA(155-254) Recombinant Protein has potential applications in both basic research and drug development. Due to its role in chromatin remodeling and gene expression regulation, this protein is a promising drug target for diseases that involve dysregulation of these processes, such as cancer and neurodegenerative disorders. In fact, studies have shown that mutations in the HIRA gene are associated with certain types of cancer, highlighting the importance of this protein in disease development.

In basic research, the truncated form of HIRA can be used to study the specific functions of the PHD finger domain, as well as its interactions with other proteins and complexes. This can provide valuable insights into the mechanisms of chromatin remodeling and gene expression regulation, which can aid in the development of new therapies for diseases involving these processes.

Conclusion

In summary, the HIRA protein is a crucial component of the chromatin remodeling complex and plays a vital role in regulating the structure and function of chromatin. The Human HIRA(155-254) Recombinant Protein, a truncated form of the full-length protein, retains the essential PHD finger domain and can be used as a drug target and a tool in basic research. Further studies on this protein and its interactions can provide a better understanding of chromatin remodeling and gene expression regulation, leading to potential advancements in disease treatment and management.