Human CD203c/ENPP3 HEK293T Stable Cell Line

The Human CD203c/ENPP3 HEK293T Stable Cell Line is a valuable tool for studying the structure, activity, and application of the human CD203c/ENPP3 protein. This stable cell line is derived from the HEK293T cell line, a popular choice for protein expression studies due to its high transfection efficiency and robust growth characteristics.

Structure of Human CD203c/ENPP3 Protein:

The human CD203c/ENPP3 protein, also known as Ectonucleotide Pyrophosphatase/Phosphodiesterase 3, is a type II transmembrane glycoprotein that belongs to the ENPP family. It is composed of 713 amino acids and has a molecular weight of approximately 80 kDa. The protein contains a N-terminal signal peptide, a large extracellular domain, a transmembrane domain, and a short cytoplasmic tail. The extracellular domain of CD203c/ENPP3 contains two conserved domains, the N-terminal catalytic domain and the C-terminal nucleotide-binding domain, which are essential for its enzymatic activity.

Activity of Human CD203c/ENPP3 Protein:

The main function of CD203c/ENPP3 protein is the hydrolysis of nucleotides, particularly pyrophosphate (PPi) and phosphodiester bonds. This enzymatic activity is crucial for the regulation of extracellular nucleotide levels, which play important roles in various physiological processes such as bone mineralization, inflammation, and immune response. CD203c/ENPP3 has been shown to be highly expressed in immune cells, including monocytes, macrophages, and dendritic cells, and has been implicated in the regulation of immune cell functions.

Application of Human CD203c/ENPP3 HEK293T Stable Cell Line:

The Human CD203c/ENPP3 HEK293T Stable Cell Line is a valuable tool for studying the structure and function of CD203c/ENPP3 protein. This cell line is stably transfected with a plasmid containing the full-length cDNA of human CD203c/ENPP3, allowing for the constitutive expression of the protein. The stable expression of CD203c/ENPP3 in this cell line ensures a consistent and reliable source of the protein for various applications.

One of the main applications of this stable cell line is in flow cytometry, a powerful technique for analyzing and sorting cells based on their physical and biochemical characteristics. The expression of CD203c/ENPP3 on the surface of the HEK293T cells allows for the detection and quantification of the protein using specific antibodies in flow cytometry experiments. This can be useful for studying the expression levels of CD203c/ENPP3 in different cell types and under different experimental conditions.

Moreover, the Human CD203c/ENPP3 HEK293T Stable Cell Line can also be used as a model system for studying the role of CD203c/ENPP3 in various biological processes. The stable expression of CD203c/ENPP3 in this cell line allows for the investigation of the protein’s enzymatic activity and its effects on cellular functions. This can provide valuable insights into the role of CD203c/ENPP3 in immune cell regulation and other physiological processes.

Furthermore, the Human CD203c/ENPP3 HEK293T Stable Cell Line can serve as a useful tool for drug discovery and development. CD203c/ENPP3 has been identified as a potential therapeutic target for various diseases, including autoimmune disorders and cancer. The stable expression of CD203c/ENPP3 in this cell line can facilitate the screening of potential drug candidates that target this protein, leading to the development of novel therapies.

In conclusion, the Human CD203c/ENPP3 HEK293T Stable Cell Line is a valuable resource for studying the structure, activity, and application of the human CD203c/ENPP3 protein.