Introduction to Human CDH17/Cadherin-17 HEK293T Stable Cell Line
Human CDH17, also known as Cadherin-17, is a transmembrane glycoprotein that belongs to the cadherin superfamily. It is expressed in various tissues including the liver, pancreas, and gastrointestinal tract. CDH17 plays a crucial role in cell adhesion, migration, and signaling, making it an important therapeutic target in various diseases. In this article, we will discuss the structure, activity, and application of the Human CDH17/Cadherin-17 HEK293T Stable Cell Line.
Structure of Human CDH17/Cadherin-17
The Human CDH17 gene is located on chromosome 8 and consists of 16 exons. The protein has a molecular weight of approximately 100 kDa and is composed of five extracellular cadherin domains, a transmembrane domain, and a cytoplasmic domain. The extracellular cadherin domains are responsible for calcium-dependent homophilic interactions with other cadherin molecules, while the cytoplasmic domain interacts with cytoskeletal proteins, mediating cell adhesion and signaling.
Activity of Human CDH17/Cadherin-17
CDH17 is involved in various cellular processes, including cell adhesion, migration, and signaling. It plays a critical role in maintaining the integrity of epithelial tissues by mediating cell-cell adhesion. CDH17 also promotes cell migration and invasion by interacting with other cadherins and extracellular matrix proteins. In addition, CDH17 has been shown to regulate cell signaling pathways, such as the Wnt/β-catenin pathway, which is important for cell proliferation and differentiation.
Application of Human CDH17/Cadherin-17 HEK293T Stable Cell Line
The Human CDH17/Cadherin-17 HEK293T Stable Cell Line is a valuable tool for studying the function and regulation of CDH17. This cell line is generated by transfecting human embryonic kidney (HEK293T) cells with a plasmid containing the CDH17 gene. The stable expression of CDH17 in these cells allows for the study of its role in cell adhesion, migration, and signaling.
One of the main applications of the Human CDH17/Cadherin-17 HEK293T Stable Cell Line is in flow cytometry. Flow cytometry is a powerful technique used to analyze and quantify the expression of cell surface markers. The stable expression of CDH17 in HEK293T cells allows for the isolation and characterization of CDH17-expressing cells, which can be used for further downstream analysis.
Another important application of this cell line is in drug discovery and development. CDH17 has been identified as a potential therapeutic target in various diseases, including cancer and inflammatory bowel disease. The Human CDH17/Cadherin-17 HEK293T Stable Cell Line can be used to screen for compounds that can modulate the activity of CDH17, leading to the development of novel therapeutic agents.
In conclusion, the Human CDH17/Cadherin-17 HEK293T Stable Cell Line is a valuable tool for studying the structure, activity, and function of CDH17. Its stable expression in HEK293T cells allows for the investigation of CDH17 in various cellular processes and its potential as a therapeutic target. With the advancement of research in this field, this cell line is expected to play an important role in the development of new treatments for diseases where CDH17 is involved.
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