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Immobilized Human CD42b-GP1BA recombinant protein, C-His Tag (cat. No.PX-P4904) at 0.5µg/mL (100µL/well) can bind Human CD42b/GP1BA Monoclonal Antibody (cat. No.ARO-A15818) in indirect ELISA with Goat Anti-Human IgG secondary antibody coupled with HRP measured by OD450 giving an EC50 at 103.8M.
Human peripheral blood monocytes were stained with an irrelevant antibody (Blue Histogram) or Human CD42b/GP1BA Monoclonal Antibody (ARO-A15818 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using Goat Anti-Mouse IgG Polyclonal Antibody, FITC (PTX17818) and cells analysed on a NovoCyte Flow Cytometer.
Human CD42b/GP1BA Monoclonal Antibody(ARO-A15818) was used at 1(ug/mL) concentration to detect Human CD42b/GP1BA Recombinant Protein, C-His Tag(PX-P6722) in Western Blot. The detection was compared to a negative control (lane 2).
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