Introduction to Human CDH6/Cadherin-6 HEK293T Stable Cell Line
Human CDH6, also known as Cadherin-6, is a transmembrane protein that belongs to the cadherin family of cell adhesion molecules. It is encoded by the CDH6 gene and is expressed in various tissues, including the brain, heart, and kidney. CDH6 plays a crucial role in cell-cell adhesion and is involved in several cellular processes, such as cell migration, proliferation, and differentiation.
The Human CDH6/Cadherin-6 HEK293T Stable Cell Line is a cell line that stably expresses the human CDH6 gene in the HEK293T cell background. This cell line is a valuable tool for studying the structure, activity, and applications of CDH6 in various research areas, including cancer, developmental biology, and neuroscience.
Structure of Human CDH6/Cadherin-6
The CDH6 gene is located on chromosome 5 and consists of 16 exons that encode a 790 amino acid protein. The protein has a molecular weight of approximately 88 kDa and is composed of five extracellular cadherin domains, a transmembrane domain, and a cytoplasmic domain. The extracellular cadherin domains are responsible for mediating cell-cell adhesion, while the cytoplasmic domain interacts with intracellular signaling molecules to regulate cellular processes.
Activity of Human CDH6/Cadherin-6
CDH6 is a calcium-dependent cell adhesion molecule that plays a crucial role in maintaining tissue integrity and regulating cell behavior. It mediates cell-cell adhesion through homophilic interactions, where the extracellular domains of two CDH6 proteins from adjacent cells bind to each other. This interaction forms a strong adhesion complex that stabilizes cell-cell contacts and facilitates tissue formation and maintenance.
Aside from its role in cell adhesion, CDH6 has also been implicated in several cellular processes, including cell migration, proliferation, and differentiation. It has been shown to regulate cell migration during embryonic development and wound healing. In addition, CDH6 has been found to play a role in the proliferation and differentiation of neural stem cells, suggesting its involvement in neurodevelopment and neurogenesis.
Application of Human CDH6/Cadherin-6 HEK293T Stable Cell Line
The Human CDH6/Cadherin-6 HEK293T Stable Cell Line is a valuable tool for studying the function of CDH6 in various research areas. One of its main applications is in cancer research, as CDH6 has been found to be dysregulated in several types of cancer, including breast, lung, and ovarian cancer. The stable expression of CDH6 in this cell line allows for the investigation of its role in cancer progression and metastasis.
Moreover, the Human CDH6/Cadherin-6 HEK293T Stable Cell Line can also be used in developmental biology research to study the role of CDH6 in tissue formation and maintenance. The stable expression of CDH6 in this cell line enables the manipulation of its expression levels, which can provide insights into its function during embryonic development and tissue regeneration.
Additionally, the stable expression of CDH6 in this cell line makes it a useful tool in neuroscience research. CDH6 has been found to be expressed in the developing and adult brain, and its dysregulation has been linked to various neurological disorders, such as autism and schizophrenia. The Human CDH6/Cadherin-6 HEK293T Stable Cell Line can be used to study the role of CDH6 in neuronal development and function, as well as its potential as a therapeutic target for neurological disorders.
Flow Cytometry Analysis of Human CDH6/Cadherin-6 HEK293T Stable Cell Line
Flow cytometry is a powerful technique that allows for the quantitative analysis of cell surface proteins.
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