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Mouse CCL2 recombinant protein(cat. No.PX-P6461) at 0.5µg/mL (100µL/well) can bind InVivoMAb Anti-Human CCL2/MCP-1 (Iv0077) in indirect ELISA with Goat secondary antibody measured by OD450.
Flow-cytometry using anti-human CCL2 antibody.Human peripheral blood lymphocytes were stained with an irrelevant antibody (Blue Histogram) or an anti-human CCL2 antibody monoclonal antibody (Catalog # ARO-A13297 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody (Catalog # PTX18862) and cells analysed on a NovoCyte Flow Cytometer.
Flow-cytometry using anti-human CCL2 antibody.A549 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CCL2 antibody monoclonal antibody (Catalog # ARO-A13297 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody (Catalog # PTX18862) and cells analysed on a NovoCyte Flow Cytometer.
Mouse CCL2 recombinant protein(cat. No.PX-P6461) can bind InVivoMAb Anti-Human CCL2/MCP-1 (Iv0077) in Western Blot Assay as detected on gel analysis.
InVivoMAb Anti-Human CCL2/MCP-1 (Iv0077), on SDS-PAGE. The gel was stained overnight with Coomassie Blue. The purity of the antibody is greater than 95%.
PX-P6461
ARO-A11121
KPTX180
ARO-A11123
ARO-A11126
ARO-A11127
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